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. 1986 Jun;321(6071):674-9.
doi: 10.1038/321674a0.

Fluorescence detection in automated DNA sequence analysis

L M Smith, J Z Sanders, R J Kaiser, P Hughes, C Dodd, C R Connell, C Heiner, S B Kent, L E Hood

Fluorescence detection in automated DNA sequence analysis

L M Smith et al. Nature. 1986 Jun.

Abstract

We have developed a method for the partial automation of DNA sequence analysis. Fluorescence detection of the DNA fragments is accomplished by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymatic DNA sequence analysis. A different coloured fluorophore is used for each of the reactions specific for the bases A, C, G and T. The reaction mixtures are combined and co-electrophoresed down a single polyacrylamide gel tube, the separated fluorescent bands of DNA are detected near the bottom of the tube, and the sequence information is acquired directly by computer.

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