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. 1986 Jun 11;14(11):4617-24.
doi: 10.1093/nar/14.11.4617.

Expression in mammalian cells of a gene from Streptomyces alboniger conferring puromycin resistance

J A Vara, A Portela, J Ortín, A Jiménez
Free PMC article

Expression in mammalian cells of a gene from Streptomyces alboniger conferring puromycin resistance

J A Vara et al. Nucleic Acids Res. .
Free PMC article

Abstract

The gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger has been cloned next to the SV40 early promoter in a mammalian cells-Escherichia coli shuttle vector. When this construction was introduced into VERO cells it expressed the relevant enzymic activity. Moreover, the puromycin N-acetyl transferase gene has been used as a dominant marker for the selection of transformed mammalian cells able to grow in the presence of the antibiotic.

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