Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2023:2667:169-179.
doi: 10.1007/978-1-0716-3199-7_13.

Genetic Mouse Models of Pneumocystis Pneumonia

J Claire Hoving  1   2   3 , Ferris T Munyonho  4 , Jay K Kolls  5
Affiliations
Review

Genetic Mouse Models of Pneumocystis Pneumonia

J Claire Hoving et al. Methods Mol Biol. 2023.

Abstract

Pneumocystis jirovecii causes pneumonia in immunocompromised patients. A major challenge in drug susceptibility testing and in understanding host/pathogen interactions is that Pneumocystis spp. are not viable in vitro. Continuous culture of the organism is not currently available, and therefore, developing new drug targets is very limited. Due to this limitation, mouse models of Pneumocystis pneumonia have proven to be an invaluable resource to researchers. In this chapter, we provide an overview of selected methods used in mouse models of infection including, in vivo Pneumocystis murina propagation, routes of transmission, genetic mouse models available, a P. murina life form-specific model, a mouse model of PCP immune reconstitution inflammatory syndrome (IRIS), and the experimental parameters associated with these models.

Keywords: Immune response; Immunocompromised; Lung fungal infection; Pneumocystis murina; Rag1-deficient.

PubMed Disclaimer

References

    1. Ma L, Chen Z, Huang D et al (2016) Genome analysis of three Pneumocystis species reveals adaptation mechanisms to life exclusively in mammalian hosts. Nat Commun 7:10740 - DOI - PubMed - PMC
    1. Cushion MT, Tisdale-Macioce N, Sayson SG et al (2021) The persistent challenge of Pneumocystis growth outside the mammalian lung: Past and future approaches. Front Microbiol 12:681474 - DOI - PubMed - PMC
    1. Shellito J, Suzara VV, Blumenfeld W et al (1990) A new model of Pneumocystis carinii infection in mice selectively depleted of helper T lymphocytes. J Clin Invest 85:16861693 - DOI
    1. Hughes WT (1982) Natural mode of acquisition for de novo infection with Pneumocystis carinii. J Infect Dis 145:842–848 - DOI - PubMed
    1. Powles MA, McFadden DC, Pittarelli LA et al (1992) Mouse model for Pneumocystis carinii pneumonia that uses natural transmission to initiate infection. Infect Immun 4:1397–1400 - DOI

LinkOut - more resources