Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 May 5;22(1):94.
doi: 10.1186/s12934-023-02106-1.

Exploring the antimicrobial, antiviral, antioxidant, and antitumor potentials of marine Streptomyces tunisiensis W4MT573222 pigment isolated from Abu-Qir sediments, Egypt

Walaa M Ibrahim  1 , Zakia A Olama  2 , G M Abou-Elela  3 , Heba S Ramadan  2 , Ghada E Hegazy  3   4 , Dalia El S El Badan  2   5
Affiliations

Exploring the antimicrobial, antiviral, antioxidant, and antitumor potentials of marine Streptomyces tunisiensis W4MT573222 pigment isolated from Abu-Qir sediments, Egypt

Walaa M Ibrahim et al. Microb Cell Fact. .

Abstract

Due to the therapeutic importance of microbial pigments, these pigments are receiving the attention of researchers. In this present study 60 isolates were isolated from sediments of Abu-Qir coast of the Mediterranean sea, Alexandria, Egypt, out of which 12 were considered as pigmented actinomycetes. Streptomyces sp. W4 was characterized by small round green pigmented colonies when grown on starch-casein agar medium. The green pigment was extracted using a mixture of acetone-methanol (7:3 v/v). The antimicrobial, antioxidant, antiviral, and anticancer activities of the green pigment produced by Streptomyces sp.W4 were investigated. The pigment was characterized using FTIR, Raman spectroscopy, EDX and GC-MS. The results revealed that the pigment has antibacterial and antifungal activity and also showed inhibition of HAV 78% but its antiviral activity against the Adenovirus was weak. The results proved the safety of the pigment toward normal cells and anticancer activity against three different cancer cell lines HepG-2 (liver cancer cell line), A549 (lung cancer cell line), and PAN1 (pancreas cancer cell line). The pigment was combined with 9 antibiotics and then tested against the Gram-negative bacterium Enterococcus faecalis using disc diffusion bioassay. LEV showed an antagonistic effect, while CXM and CIP showed a synergistic effect.

Keywords: Antibacterial; Marine actinomycetes; Pigments; Secondary metabolites.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Scanning electron micrograph of the selected isolate W4 showing branching mycelia (A), mycelia with spores (B)
Fig. 2
Fig. 2
Phylogenetic tree, based on the 16S rRNA gene sequence, isolate W4 and the closest relatives. The tree was obtained by the neighbor-joining method
Fig. 3
Fig. 3
UV–Visible spectra (A), Fourier-Transform Infrared spectrum (B) and Raman spectra (C) of the tested pigment
Fig. 4
Fig. 4
Energy Dispersive X-ray analysis (EDX) of Streptomyces tunisiensisW4MT573222 pigment
Fig. 5
Fig. 5
GC–MS chart analysis of Streptomyces tunisiensis W4MT573222 pigment
Fig. 6
Fig. 6
A Antibacterial activity of the Streptomyces tunisiensis W4MT573222 pigment against Enterococcus faecalis ATCC29212. B Antifungal activity of Streptomyces tunisiensis W4MT573222pigment against Fusarium solani ATCC10557, 50 µL (B), 100 µL (C) and control (A). Pig refers to pigment; d refers to day
Fig. 7
Fig. 7
A1 Antiviral effect of Streptomyces tunisiensisW4MT573222 pigment at a concentration of 625 µg/mL against HAV(all the actions done through HAV have been decreased by 78%). A2 Antiviral effect of acyclovir at a concentration of 62.5 µg/mL against HAV. B1 Antiviral effect of Streptomyces tunisiensisW4MT573222 pigment at a concentration of 625 µg/mL against Adenovirus (all the actions done through Adenovirus have been decreased by 13%). B2 Antiviral effect of acyclovir at a concentration of 62.5 µg/mL against Adenvirus
Fig. 8
Fig. 8
Antiviral effects of Acyclovir at different concentrations against HAV and Adenovirus
Fig. 9
Fig. 9
Antiviral effects of Streptomyces tunisiensis W4MT573222 pigment at different concentrations against HAV and Adenovirus
Fig. 10
Fig. 10
Effect of Streptomyces tunisiensis W4MT573222 pigment against HepG2 cells showed complete loss of the monolayer sheet of cells at concentration 10,000 µg, while at concentration 5000 µg showed partial loss of the sheet (1) and rounding cells without granulation (2) appearance of normal sheet was observed at concentration 2500 µg with same rounding cells (3) while at the rest of concentration the normal sheet was observed
Fig. 11
Fig. 11
Effect of Streptomyces tunisiensis W4MT573222 pigment on HepG2 cells at different concentrations
Fig. 12
Fig. 12
Effect of Streptomyces tunisiensis W4MT573222 pigment against A549 cells showed complete loss of the monolayer sheet of cells at concentration 10,000 µg, while at concentration 5000ug showed partial loss of the sheet (1) and shrinkage of cells (2) appearance of normal sheet was observed at concentration 2500 µg with same rounding cells (3) and shrinkage cells and partial loss of the monolayer sheet, same rounded cells were appeared at concentration of 1250 µg/mL, while at the rest of concentration the normal sheet was observed
Fig. 13
Fig. 13
Effect of Streptomyces tunisiensis W4MT573222 pigment against A549 cells at different concentrations
Fig. 14
Fig. 14
Effect of Streptomyces tunisiensisW4MT573222pigment against PANC1 cells showed complete loss of the monolayer sheet of cells at concentration 10,000 µg, while at concentration 5000 µg showed partial loss of the sheet (1) and shrinkage of cells (2) appearance of normal sheet was observed at concentration 2500 µg without any cytopathic effect, while at the rest of concentration the normal sheet was observed
Fig. 15
Fig. 15
Effect of Streptomyces tunisiensis W4MT573222 pigment against PAN1 cells at different concentrations
Fig. 16
Fig. 16
Effect of the combined action of some commercial antibiotics and Streptomyces tunisiensis W4MT573222 extracted pigment against Enterococcus faecalis ATCC29212
See this image and copyright information in PMC

References

    1. Sreenivasa N, Muthuraj R, Bidhayak C, Meghashyama PB, Pallavi SS, Shashiraj KN, Halaswamy HM, Dhanyakumara SB, Dattatraya A, Hagedc K. A potential bioactive secondary metabolites and antimicrobial efficacy of Streptomyces thermocarboxydus strain KSA-2, isolated from Kali River, Karwar. Curr Res Microbiol Infect. 2020;1(1):5–13. doi: 10.31559/CRMI2020.1.1.2. - DOI
    1. Okami Y, Hotta K. Search and discovery of new antibiotics. In: Good Fellow M, Williams ST, Mordarski M, editors. Actinomycetes in biotechnology. London: Academic press; 1988. pp. 37–67.
    1. Atta HM, Dabour SM, Desoukey SG. Sparsomycin antibiotic production by Streptomyces sp. AZ-NIOFD1: taxonomy, fermentation, purification and biological activities. Am-Eur J Agric Environ Sci. 2009;5(3):368–377.
    1. Sateesh V, Naikpatil S, Rathod JL. Selective isolation and antimicrobial activity of rare actinomycetes from mangrove sediment of Karwar. J Ecobiotechnol. 2011;3(10):48–53.
    1. Ravikumar S, Inbaneson SJ, Uthiraselvam M, Priya SR, Ramu A, Banerjee MB. Diversity of endophytic actinomycetes from Karangkadu mangrove ecosystem and its antibacterial potential against bacterial pathogens. J Pharm Res. 2011;4(1):294–296.

MeSH terms

Supplementary concepts

LinkOut - more resources