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. 2023:2657:253-284.
doi: 10.1007/978-1-0716-3151-5_19.

Long-Read Metagenomics and CAZyme Discovery

Alessandra Ferrillo  1 , Carl Mathias Kobel  2 , Arturo Vera-Ponce de León  1   2 , Sabina Leanti La Rosa  1 , Benoit Josef Kunath  3 , Phillip Byron Pope  1   2 , Live Heldal Hagen  4
Affiliations

Long-Read Metagenomics and CAZyme Discovery

Alessandra Ferrillo et al. Methods Mol Biol. 2023.

Abstract

Microorganisms play a primary role in regulating biogeochemical cycles and are a valuable source of enzymes that have biotechnological applications, such as carbohydrate-active enzymes (CAZymes). However, the inability to culture the majority of microorganisms that exist in natural ecosystems restricts access to potentially novel bacteria and beneficial CAZymes. While commonplace molecular-based culture-independent methods such as metagenomics enable researchers to study microbial communities directly from environmental samples, recent progress in long-read sequencing technologies are advancing the field. We outline key methodological stages that are required as well as describe specific protocols that are currently used for long-read metagenomic projects dedicated to CAZyme discovery.

Keywords: Assembly; Binning; Carbohydrate-active enzymes; Long-read metagenomics; Microbial communities.

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References

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