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. 2023 Mar-Apr;27(2):189-194.
doi: 10.4103/jisp.jisp_109_22. Epub 2023 Mar 4.

Elemental analysis of various demineralized tooth graft by EDX-720 XRF: An in vitro study

M Amudha Saravanan  1 , R Renuka Devi  2 , H Esther Nalini  2
Affiliations

Elemental analysis of various demineralized tooth graft by EDX-720 XRF: An in vitro study

M Amudha Saravanan et al. J Indian Soc Periodontol. 2023 Mar-Apr.

Abstract

Background: The periodontal regeneration is the most challenging process which involves regeneration of both hard and soft tissues. There are various biomaterials available for this purpose. Tooth graft can be used as a regenerative material in the field of periodontics and implantology. The tooth graft with 70% demineralization is considered to be a better option for periodontal regeneration. Different methods are employed for demineralization of tooth using various acids.

Materials and methods: This study was designed as an in vitro study. Totally 60 teeth were collected and categorized into four groups. Groups I, II, and III teeth were demineralized in 2% nitric acid, 0.6 M hydrochloric acid, and 17% ethylenediaminetetraacetic acid (EDTA), respectively. The Group IV teeth were left undemineralized. The elements present in the various tooth graft groups after demineralization were analyzed by EDX-720 XRF Instrument.

Results: The EDTA demineralized tooth graft has higher level of Calcium, Strontium, and Zinc content compared to other tooth graft groups and it was statistically significant with P = 0.001.

Conclusion: The EDTA demineralized tooth graft can serve as better graft than 2% nitric acid and 0.6M hydrochloric acid demineralized and undemineralized tooth grafts.

Keywords: EDX-720 XRF; Ethylenediaminetetraacetic acid; X-ray fluorescence spectroscopy; periodontal regeneration; tooth graft.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Steps in study process
Figure 2
Figure 2
Tooth graft
Figure 3
Figure 3
Acids for demineralization
Figure 4
Figure 4
EDX 720 XRF
Figure 5
Figure 5
XRF quantitative result for HNO3 demineralized tooth graft elements
Figure 6
Figure 6
XRF quantitative result for Hcl demineralized tooth graft elements
Figure 7
Figure 7
XRF quantitative result for EDTA demineralized tooth graft elements
Figure 8
Figure 8
XRF quantitative result for undemineralized tooth graft elements
Figure 9
Figure 9
Comparison of mean calcium percentage between tooth grafts
Figure 10
Figure 10
Comparison of mean strontium percentage between tooth grafts
Figure 11
Figure 11
Comparison of mean zinc percentage between tooth grafts
See this image and copyright information in PMC

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