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. 2023 Aug;113(4):e22022.
doi: 10.1002/arch.22022. Epub 2023 May 8.

Candidate odorant-binding protein and chemosensory protein genes in the turnip aphid Lipaphis erysimi

Yinhui Kuang  1 , Chaozhi Shangguan  1 , Sichen Yuan  1 , Qiaoqin Zhang  1 , Ziying Qiu  1 , Liwei Gao  1 , Xiudao Yu  1
Affiliations

Candidate odorant-binding protein and chemosensory protein genes in the turnip aphid Lipaphis erysimi

Yinhui Kuang et al. Arch Insect Biochem Physiol. 2023 Aug.

Abstract

The turnip aphid, Lipaphis erysimi Kaltenbach, inflicts heavy damage on cruciferous crops worldwide. In these insects, olfactory perception is crucial for mating, host location, and oviposition. Both odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are responsible for the delivery of host odorants and pheromones during initial molecular interactions. In this study, antennal and body transcriptomes of L. erysimi were generated through the deep sequencing of RNA libraries. A dataset of 11 LeryOBP and four LeryCSP transcripts was identified among assembled unigenes and subjected to sequence analysis. Phylogenetic analysis found a one-to-one orthologous relationship between LeryOBP/LeryCSP and its corresponding homologs from other aphid species. Further quantitative real-time PCR analyses across developmental stages and tissues showed that five LeryOBP genes (i.e., LeryGOBP, LeryOBP6, LeryOBP7, LeryOBP9, and LeryOBP13) and LeryCSP10 were specifically or significantly elevated in the antennae compared with other tissues. Moreover, two transcripts (i.e., LeryGOBP and LeryOBP6) exhibited remarkably higher expression levels in alate aphids, implying their potentially functional role in the perception of new host plant locations. These results present the identification and expression of OBP/CSP genes in L. erysimi, providing valuable insights into their putative role in olfactory signal transduction.

Keywords: Lipaphis erysimi; antennal transcriptome; chemosensory protein; gene expression; odorant-binding protein.

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