Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Jul;50(7):5535-5545.
doi: 10.1007/s11033-023-08454-w. Epub 2023 May 8.

Biochemical and SSR based molecular characterization of elite rice varieties for straw lignocellulose

Abinash Mishra  1 , Jyoti Prakash Sahoo  1 , Bishnupriya Swain  2 , Spandan Nanda  3 , Tapash Kumar Mishra  1 , Sanat Kumar Dwibedi  1 , Bandita Jena  1 , Banshidhar Pradhan  1 , Manas Ranjan Parida  3 , Pradip Kumar Jena  4 , Saubhagya Manjari Samantaray  4 , Debiprasad Samantaray  4 , Mahendra Kumar Mohanty  3 , Manasi Dash  5
Affiliations

Biochemical and SSR based molecular characterization of elite rice varieties for straw lignocellulose

Abinash Mishra et al. Mol Biol Rep. 2023 Jul.

Abstract

Background: Lignocellulosic biomass from rice straw possesses enormous potential in generating bioenergy thereby reducing the dependence of human on non-renewable fuel sources. Developing rice varieties of such calibre necessitates biochemical characterization as well as assessing the presence of genetic diversity among the rice genotypes with respect to cellulose content.

Methods and results: Forty-three elite rice genotypes were selected for biochemical characterization and SSR marker-based genetic fingerprinting. For genotyping, 13 cellulose synthase specific polymorphic markers were used. The diversity analysis was performed using TASSEL 5.0 and GenAlE × 6.51b2, software program. Of the 43 rice varieties, CR-Dhan-601, CR-Dhan-1014, Mahanadi, Jagabandhu, Gouri, Samanta and Chandrama were found to possess desirable lignocellulosic composition with respect to harnessing green fuels. The marker OsCESA-1.3 expressed the highest PIC (0.640), while the marker OsCESA-6.3 of lowest PIC (0.128). A moderate average estimate (0.367) of PIC was observed under current set of genotypes and marker system. The dendrogram analysis grouped the rice genotypes into two principal clusters i.e., cluster I and II. Cluster-II is monogenetic, while cluster-I is having 42 genotypes.

Conclusions: The moderate level of both PIC and H average estimates indicate the narrow genetic bases of the germplasms. The varieties falling under different clusters possessing desirable lignocellulosic composition can be used in a hybridization programme to develop bioenergy efficient varieties. The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation. This study helped in identification of suitable dual purpose rice varieties for biofuel production without compromising food security.

Keywords: Characterization; Dendrogram; Diversity; Fingerprinting; Lignocellulose; Rice.

PubMed Disclaimer

References

    1. Dash M, Mishra A, Mohanty MK (2021) Breeding Rice for Sustainable Bioenergy Production. In Integrative Advances in Rice Research. IntechOpen. London, United Kingdom. pp 109–127. https://doi.org/10.5772/intechopen.98572
    1. Ali I, Bahaitham H, Naebulharam R (2017) A comprehensive kinetics study of coconut shell waste pyrolysis. Bioresour Technol 235:1–11. https://doi.org/10.1016/j.biortech.2017.03.089 - DOI - PubMed
    1. Kaur R, Gera P, Jha MK, Bhaskar T (2018) Pyrolysis kinetics and thermodynamic parameters of castor (Ricinus communis) residue using thermogravimetric analysis. Bioresour Technol 250:422–428. https://doi.org/10.1016/j.biortech.2017.11.077 - DOI - PubMed
    1. Pal DB, Tiwari AK, Srivastava N, Hashem A, Abd Allah EF (2021) Thermal studies of biomass obtained from the seeds of Syzygium cumini and Cassia fistula L. and peel of Cassia fistula L. fruit. Biomass Convers Biorefin 1–12. https://doi.org/10.1007/s13399-021-01492-z
    1. Bhattacharyya P, Bhaduri D, Adak T, Munda S, Satapathy BS, Dash PK, Padhy SR, Pattanayak A, Routray S, Chakraborti MJ, Baig MJ (2020) Characterization of rice straw from major cultivars for best alternative industrial uses to cutoff the menace of straw burning. Ind Crop Prod 143:111919. https://doi.org/10.1016/j.indcrop.2019.111919 - DOI

LinkOut - more resources