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. 2023 Jun;54(2):761-768.
doi: 10.1007/s42770-023-00987-3. Epub 2023 May 9.

MALDI-TOF as a tool for microbiological monitoring in areas considered aseptic

Marlu Lopes Guimarães  1 , Maximino Alencar Bezerra Júnior  1 , Vitor Martins de Almeida  2 , Wilson Vicente Souza Pereira  3
Affiliations

MALDI-TOF as a tool for microbiological monitoring in areas considered aseptic

Marlu Lopes Guimarães et al. Braz J Microbiol. 2023 Jun.

Abstract

To maintain asepsis in production environments, contamination must be constantly controlled. To this end, microbiological monitoring is constantly used with the objective of evaluating the incidence of microorganisms prevalent in the sampling of air, surface, and people, in the area of an environment considered aseptic, isolated, and identified using the rapid and automated phenotypic microbiological methodology, highlighting the MALDI-TOF mass spectrometry analysis technique (MS), being identified at the level of genus and/or species. For that purpose, microbiological control of environmental monitoring of environments considered aseptic in a pharmaceutical industry was conducted for 12 months. The isolated microorganisms were identified using the mass spectrometry identification method (MALDI-TOF). In area classification A, the most prevalent microorganisms were bacteria in the sampling person. The microbial population was composed of bacteria of the genus Micrococcus sp. and Staphylococcus sp. Based on the results, it is possible to observe that in an environment where the process requires human operations, possible microbial contamination is inevitable and requires the identification of microorganisms at least at the level of species and/or genus. The microorganisms identified and found in the sampling of the aseptic environment must be evaluated with frequency to ensure that the productive environment guarantees the quality of the product produced.

Keywords: Clean rooms; Mass spectrometry; Microbiological control; Microorganisms.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Number of microorganisms founded on sampling of surfaces (A) and active air (B) on a A-degree clean area during the study period
Fig. 2
Fig. 2
Number of microorganisms founded on sampling people on a A-degree clean area during the study period sorted by genus
Fig. 3
Fig. 3
Number of microorganisms founded on sampling people on a A-degree clean area during the study period sorted by genus
See this image and copyright information in PMC

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