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[Preprint]. 2023 Apr 26:2023.04.25.538338.
doi: 10.1101/2023.04.25.538338.

Structure determination of a DNA crystal by MicroED

Alison Haymaker, Andrey A Bardin, Tamir Gonen, Michael W Martynowycz, Brent L Nannenga

Structure determination of a DNA crystal by MicroED

Alison Haymaker et al. bioRxiv. .

Update in

  • Structure determination of a DNA crystal by MicroED.
    Haymaker A, Bardin AA, Gonen T, Martynowycz MW, Nannenga BL. Haymaker A, et al. Structure. 2023 Dec 7;31(12):1499-1503.e2. doi: 10.1016/j.str.2023.07.005. Epub 2023 Aug 3. Structure. 2023. PMID: 37541248 Free PMC article.

Abstract

Microcrystal electron diffraction (MicroED) is a powerful tool for determining high-resolution structures of microcrystals from a diverse array of biomolecular, chemical, and material samples. In this study, we apply MicroED to DNA crystals, which have not been previously analyzed using this technique. We utilized the d(CGCGCG) 2 DNA duplex as a model sample and employed cryo-FIB milling to create thin lamella for diffraction data collection. The MicroED data collection and subsequent processing resulted in a 1.10 Å resolution structure of the d(CGCGCG) 2 DNA, demonstrating the successful application of cryo-FIB milling and MicroED to the investigation of nucleic acid crystals.

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