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. 2023 Aug;23(15):e2200437.
doi: 10.1002/pmic.202200437. Epub 2023 May 12.

Global profiling of lysine lactylation in human lungs

Ye-Hong Yang  1 , Qiao-Chu Wang  1 , Jie Kong  1 , Jun-Tao Yang  1 , Jiang-Feng Liu  1
Affiliations

Global profiling of lysine lactylation in human lungs

Ye-Hong Yang et al. Proteomics. 2023 Aug.

Abstract

Lactate is closely related to various cellular processes, such as angiogenesis, responses to hypoxia, and macrophage polarization, while regulating natural immune signaling pathways and promoting neurogenesis and cognitive function. Lysine lactylation (Kla) is a novel posttranslational modification, the examination of which may lead to new understanding of the nonmetabolic functions of lactate and the various physiological and pathological processes in which lactate is involved, such as infection, tumorigenesis and tumor development. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), researchers have identified lactylation in human gastric cancer cells and some other species, but no research on lactylation in human lungs has been reported. In this study, we performed global profiling of lactylation in human lungs under normal physiological conditions, and 724 Kla sites in 451 proteins were identified. After comparing the identified proteins with those reported in human lactylation datasets, 141 proteins that undergo lactylation were identified for the first time in this study. Our work expands the database on human lactylation and helps advance the study on lactylation function and regulation under physiological and pathological conditions.

Keywords: LC-MS/MS; Lysine lactylation; human lung; post-translational modification.

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References

REFERENCES

    1. Sharma, N. K., & Pal, J. K. (2021). Metabolic ink lactate modulates epigenomic landscape: A concerted role of pro-tumor microenvironment and macroenvironment during carcinogenesis. Current Molecular Medicine, 21, 177-181.
    1. Xin, Q., Wang, H., Li, Q., Liu, S., Qu, K., Liu, C., & Zhang, J. (2022). Lactylation: A passing fad or the future of posttranslational modification. Inflammation, 45, 1419-1429.
    1. Zhang, D., Tang, Z., Huang, H., Zhou, G., Cui, C., Weng, Y., Liu, W., Kim, S., Lee, S., Perez-Neut, M., Ding, J., Czyz, D., Hu, R., Ye, Z., He, M., Zheng, Y. G, Shuman, H. A., Dai, L., Ren, B., … Zhao, Y. (2019). Metabolic regulation of gene expression by histone lactylation. Nature, 574, 575-580.
    1. Chen, A.-N., Luo, Y., Yang, Y.-H., Fu, J.-T., Geng, X.-M., Shi, J.-P., & Yang, J. (2021). Lactylation, a novel metabolic reprogramming code: Current status and prospects. Frontiers in Immunology, 12, 688910.
    1. Yu, J., Chai, P., Xie, M., Ge, S., Ruan, J., Fan, X., & Jia, R. (2021). Histone lactylation drives oncogenesis by facilitating m6 A reader protein YTHDF2 expression in ocular melanoma. Genome Biology, 22, 85.

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