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. 2023 May 13;23(1):128.
doi: 10.1186/s12866-023-02878-x.

Differences and biocontrol potential of haustorial endophytic fungi from Taxillus Chinensis on different host plants

Li-Sha Song  1   2 , Juan Huo  1   2 , Lingyun Wan  1   2 , Limei Pan  1   2 , Ni Jiang  1   2 , Jine Fu  1   2 , Shugen Wei  3   4 , Lili He  5   6
Affiliations

Differences and biocontrol potential of haustorial endophytic fungi from Taxillus Chinensis on different host plants

Li-Sha Song et al. BMC Microbiol. .

Abstract

Background: To explore the community composition and diversity of the endophytic fungi in Taxillus chinensis, samples of the parasites growing on seven different hosts, Morus alba, Prunus salicina, Phellodendron chinense, Bauhinia purpurea, Dalbergia odorifera, Diospyros kaki and Dimocarpus longan, were isolated. The strains were identified by their morphological characteristics and their internal transcribed spacer (ITS) sequences.

Results: 150 different endophytic fungi were isolated from the haustorial roots of the seven hosts with a total isolation rate of 61.24%. These endophytic fungi were found to belong to 1 phylum, 2 classes, 7 orders, 9 families, 11 genera and 8 species. Among of them, Pestalotiopsis, Neopestalotiopsis and Diaporthe were the dominant genera, accounting for 26.67, 17.33 and 31.33% of the total number of strains, respectively. Diversity and similarity analyses showed that the endophytic fungi isolated from D. longan (H'=1.60) had the highest diversity index. The highest richness indexes were found in M. alba and D. odorifera (both 2.23). The evenness index of D. longan was the highest (0.82). The similarity coefficient of D. odorifera was the most similar to D. longan and M. alba (33.33%), while the similarity coefficient of P. chinense was the lowest (7.69%) with M. alba and D. odorifera. Nine strains showed antimicrobial activities. Among them, Pestalotiopsis sp., N. parvum and H. investiens showed significant antifungal activity against three fungal phytopathogens of medicinal plants. At the same time, the crude extracts from the metabolites of the three endophytic fungi had strong inhibitory effects on the three pathogens. Pestalotiopsis sp., N. parvum and H. investiens had the strongest inhibitory effects of S. cucurbitacearum, with inhibitory rates of 100%, 100% and 81.51%, respectively. In addition, N. parvum had a strong inhibitory effect on D. glomerata and C. cassicola, with inhibitory rates of 82.35% and 72.80%, respectively.

Conclusions: These results indicate that the species composition and diversity of endophytic fungi in the branches of T. chinensis were varied in the different hosts and showed good antimicrobial potential in the control of plant pathogens.

Keywords: Biodiversity; Inhibitory activity; Phylogeny; Plant endophytes; Taxillus chinensis.

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Conflict of interest statement

Not applicable.

Figures

Fig. 1
Fig. 1
The isolation rates of endophytic fungi from T. chinensis
Fig. 2
Fig. 2
Phylogenetic analysis of endophytic fungi from T. chinensis parasitized on different host plants based on rDNA-ITS.
Fig. 3
Fig. 3
The isolation frequency of orders (A) and genera (B) related to Taxillus chinensis parasitized on different host plants
Fig. 4
Fig. 4
The diversity and distribution of endophytic fungi isolated from Taxillus chinensis parasitized on different host plants
Fig. 5
Fig. 5
The inhibitory effects of crude extracts from fermentation products of endophytic fungi
Fig. 6
Fig. 6
Maps showing the locations of the sample collection sites
See this image and copyright information in PMC

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