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. 2023 Apr 22;28(9):3646.
doi: 10.3390/molecules28093646.

Preparation of Molecularly Imprinted Cysteine Modified Zinc Sulfide Quantum Dots Based Sensor for Rapid Detection of Dopamine Hydrochloride

Xin Zhang  1   2 , Meng Wang  1 , Yating Zhang  1 , Pan Zhao  1 , Jiamei Cai  1 , Yunjian Yao  1 , Jiarong Liang  1
Affiliations

Preparation of Molecularly Imprinted Cysteine Modified Zinc Sulfide Quantum Dots Based Sensor for Rapid Detection of Dopamine Hydrochloride

Xin Zhang et al. Molecules. .

Abstract

By combining surface molecular imprinting technology with cysteine-modified ZnS quantum dots, an elegant, molecularly imprinted cysteine-modified Mn2+: ZnS QDs (MIP@ZnS QDs) based fluorescence sensor was successfully developed. The constructed fluorescence sensor is based on a molecularly imprinted polymer (MIP) coated on the surface cysteine-modified ZnS quantum dots and used for rapid fluorescence detection of dopamine hydrochloride. The MIP@ZnS quantum dots possess the advantages of rapid response, high sensitivity, and selectivity for the detection of dopamine hydrochloride molecules. Experimental results show that the adsorption equilibrium time of MIP@ZnS QDs for dopamine hydrochloride molecules is 12 min, and it can selectively capture and bind dopamine in the sample with an imprinting factor of 29.5. The fluorescence quenching of MIP@ZnS QDs has a good linear (R2 = 0.9936) with the concentration of dopamine hydrochloride ranged from 0.01 to 1.0 μM, and the limit of detection is 3.6 nM. In addition, The MIP@ZnS QDs demonstrate good recyclability and stability and are successfully employed for detection of dopamine hydrochloride in urine samples with recoveries was 95.2% to 103.8%. The proposed MIP@ZnS QDs based fluorescent sensor provides a promising approach for food safety detection and drug analysis.

Keywords: dopamine hydrochloride; fluorescent sensor; molecular imprinting; quantum dots.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Schematic of preparation of dopamine hydrochloride molecularly imprinted cysteine-modified Mn2+: ZnS QDs-based fluorescent sensor. dopamine hydrochloride: 2.0 mg; Mn2+: ZnS QDs: 100 mg.
Figure 2
Figure 2
Fluorescence spectrum of (a) MIP@ZnS QDs elution of template molecule; (b) MIP@QDs before elution of template molecule, and (c) NIP@ZnS QDs.
Figure 3
Figure 3
(A) TEM image and (B) particle size distribution of prepared MIP@QDs.
Figure 4
Figure 4
Effect of monomer (MAA) amount on the fluorescence quenching degree of MIP@ZnS QDs.
Figure 5
Figure 5
The fluorescence intensity of (a) MIP@ZnS QDs and (b) MIP@ZnS QDs bound with dopamine hydrochloride molecule at different pH values; (c) the change of fluorescence quenching of MIP@ZnS QDs at different pH values. Dopamine hydrochloride: 0.5 μM; room temperature.
Figure 6
Figure 6
Binding kinetic curve of MIP@ZnS QDs and NIP@ZnS QDs. Dopamine hydrochloride: 0.5 μM; room temperature.
Figure 7
Figure 7
Fluorescence emission spectra of (A) MIP@ZnS QDs and (B) NIP@ ZnS QDs at different concentrations of dopamine hydrochloride; (C) Fitting curves of fluorescence quenching values (F0/F − 1) against the concentration of dopamine hydrochloride; Experimental conditions: room temperature, concentrations of dopamine hydrochloride: 0.01~1.0 μM.
Figure 8
Figure 8
Quenching constants of MIP@ZnS QDs, NIP@ ZnS QDs and imprinting factors (IF) for dopamine hydrochloride (DA-HCl), p-aminophenol (PAP), pyrocatechol and carbamide.
Figure 9
Figure 9
Recyclability test of MIP@ZnS QDs.
Figure 10
Figure 10
Fluorescence stability of MIP@ZnS QDs.
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