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. 2024 Mar;66(3):432-441.
doi: 10.1007/s12033-023-00762-7. Epub 2023 May 13.

Inhibition of TNF-α Oncogene Expression by Artemisia Annua L. Extract Against Pioglitazone Side Effects in Male Albino Mice

Silvia Botrous  1 , Ayaat Elmaghraby  2 , Samar El-Achy  3 , Yehia Mustafa  1 , Effat Badr  1 , Amany Haggag  2 , Salah Abdel-Rahman  4
Affiliations

Inhibition of TNF-α Oncogene Expression by Artemisia Annua L. Extract Against Pioglitazone Side Effects in Male Albino Mice

Silvia Botrous et al. Mol Biotechnol. 2024 Mar.

Abstract

Pioglitazone (Actos) is one of the most recent oral antidiabetic drugs for treating the second type of diabetes mellitus as a common chronic and lifelong disease, but with harmful side effects. The objective of this study is to evaluate the effectiveness of Artemisia annua L. extract against the Actos drug side effects in the male albino mice. In present study, the use of Actos drug alone induced hepatotoxicity, renal inflammation, hematological disorders and bladder cancer, which are manifested by biochemical abnormalities and histopathological changes, moreover, the severity of toxicity depends on its dose. In contrast, the concurrent treatment with both Actos drug (45 mg/kg) and Artemisia extract (4 g/kg) was effective against the harmful side effects of the Actos drug. Where, the biochemical, hematological and histopathological investigations showed that the hepatotoxicity, renal inflammation, hematological disorders and histopathological changes were improved using combination of Actos and Artemisia extract. In addition, the results of TNF-ɑ oncogene expression levels in bladder tissues were significantly decreased by about 99.99% using the mix of both Actos drug and Artemisia extract. In conclusion, these findings reveal that the Artemisia annua extract on TNF-ɑ oncogene expression level is very significant and effective natural product against harmful side effects of pioglitazone which associated with an increased risk of incident bladder cancer among people, but for application more studies must be achieved in that field.

Keywords: Actos drug; Artemisia annua; Bladder cancer; Pioglitazone; TNF-α gene.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
a, b, c and d Histological examinations of liver (a), kidney (b), urinary bladder (c) and testis (d) tissues of studied groups (A, B3, C3, D1 and E). Where, A is control group, B is group B3, C is group C3, D is group D1 and E is group E
Fig. 2
Fig. 2
Gene expression level of TNF-ɑ gene in urinary bladder tissues of studied groups in the main experiment (15 and 30 days). Where, A is control group, D1 (Actos/Artemisia) and E (Actos/Artemisia/Ferric). The values are expressed as mean ± SD (Standard Deviation) with significant at p < 0.05
See this image and copyright information in PMC

References

    1. Mizrachi E, Bernel-Mizrachi C. Diabetes mellitus and related disorders. In: Cooper DH, Krainik AJ, Lubner SJ, Reno HEL, editors. Washington manual of medical therapeutics. 32. Philadelphia: Wolters Kluwer/Lippincott Williums and Wilkins; 2007. pp. 600–623.
    1. Desouza CV, Shivaswamy V. Pioglitazone in the treatment of type 2 diabetes: Safety and efficacy review. Clinical Medicine Insights. Endocrinology and Diabetes. 2010;3:43–51. doi: 10.4137/CMED.S5372. - DOI - PMC - PubMed
    1. Belfort R, Harrison SA, Brown K, Darland C, Finch J, Hardies J, Balas B, Gastaldelli A, Tio F, Pulcini J, Berria R. A placebo-controlled trial of pioglitazone in subjects with nonalcoholic steatohepatitis. The New England Journal of Medicine. 2006;355:2297–2307. doi: 10.1056/NEJMoa060326. - DOI - PubMed
    1. Nolte MS. Pancreatic hormones and antidiabetic drugs. In: Katzung BG, Masters SB, Trevor AJ, editors. Basic and clinical pharmacology. 11. New Delhi: Tata McGraw Hill Education Private Limited; 2009. pp. 727–751.
    1. Schernthaner G, Matthews DR, Charbonnel B, Hanefeld M, Brunetti P. Quartet study group. Efficacy and safety of pioglitazone versus metformin in patients with type 2 diabetes mellitus: A doubleblind, randomized trial. Journal of Clinical Endocrinology and Metabolism. 2004;89:6068–6076. doi: 10.1210/jc.2003-030861. - DOI - PubMed