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. 2023:30:15.
doi: 10.1051/parasite/2023015. Epub 2023 May 14.

Monorchiids (Digenea, Trematoda) of fishes in the Yucatán Peninsula, Mexico, with the description of three new species based on morphological and molecular data

Leopoldo Andrade-Gómez  1 , Mirza Patricia Ortega-Olivares  2 , Brenda Solórzano-García  1 , Martín García-Varela  2 , Berenit Mendoza-Garfias  2 , Gerardo Pérez-Ponce de León  1
Affiliations

Monorchiids (Digenea, Trematoda) of fishes in the Yucatán Peninsula, Mexico, with the description of three new species based on morphological and molecular data

Leopoldo Andrade-Gómez et al. Parasite. 2023.

Abstract
in English, French

Adult specimens of monorchiids (Digenea) were collected from the intestines of the white grunt, Haemulon plumierii Lacepède (Haemulidae), and the white mullet, Mugil curema Valenciennes (Mugilidae) from five localities off the Yucatán Peninsula and one locality in the Gulf of Mexico. Some specimens were photographed and sequenced for two molecular markers, the large subunit (LSU) of nuclear rDNA and the cytochrome c oxidase subunit 1 (cox1) of mitochondrial DNA. Other specimens were processed for morphological analyses. Newly generated sequences were aligned with other sequences available in GenBank. Bayesian inference and maximum likelihood analyses were implemented using the data sets of LSU and cox1 independently. Reciprocal monophyly evidenced through phylogenetic analyses, sequence divergence values for both molecular markers, and detailed morphological analyses, including scanning electron microscopy photomicrographs, revealed three new genetic lineages, i.e., species, as parasites of M. curema. The three new species are Sinistroporomonorchis mexicanus n. sp., Sinistroporomonorchis yucatanensis n. sp., and Sinistroporomonorchis minutus n. sp. Two additional species of monorchiids were sampled, characterised molecularly, and re-described, namely Sinistroporomonorchis glebulentus (Overstreet, 1971) from the white mullet, and Alloinfundiburictus haemuli (Overstreet, 1969), from the white grunt.

Title: Monorchiidae (Digenea, Trematoda) de poissons de la péninsule du Yucatán, Mexique, avec description de trois nouvelles espèces sur la base de données morphologiques et moléculaires.

Abstract: Des spécimens adultes de Monorchiidae (Digenea) ont été collectés dans les intestins de la gorette blanche, Haemulon plumierii Lacepède (Haemulidae), et du mulet blanc, Mugil curema Valenciennes (Mugilidae) de cinq localités au large de la péninsule du Yucatán et d'une localité dans le Golfe du Mexique. Certains spécimens ont été photographiés et séquencés pour deux marqueurs moléculaires, la grande sous-unité (LSU) de l'ADNr nucléaire et la sous-unité 1 de la cytochrome c oxydase (cox1) de l'ADN mitochondrial. D'autres spécimens ont été traités pour des analyses morphologiques. Les séquences nouvellement générées ont été alignées avec d'autres séquences disponibles dans GenBank. L'inférence bayésienne et les analyses de vraisemblance maximale ont été mises en œuvre en utilisant les ensembles de données de LSU et cox1 indépendamment. La monophylie réciproque mise en évidence par des analyses phylogénétiques, des valeurs de divergence de séquence pour les deux marqueurs moléculaires et des analyses morphologiques détaillées, y compris des photomicrographies au microscope électronique à balayage, a révélé trois nouvelles lignées génétiques, c'est-à-dire des espèces, qui sont parasites de M. curema. Les trois nouvelles espèces sont Sinistroporomonorchis mexicanus n. sp., Sinistroporomonorchis yucatanensis n. sp. et Sinistroporomonorchis minutus n. sp. (Monorchiidae). Deux espèces supplémentaires de Monorchiidae ont été échantillonnées, caractérisées moléculairement et redécrites, à savoir Sinistroporomonorchis glebulentus (Overstreet, 1971) du mulet blanc et Alloinfundiburictus haemuli (Overstreet, 1969) de la gorette blanche.

Keywords: Digenea; Haemulidae; LSU; Mugilidae; SEM; cox1.

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Conflict of interest statement

Authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Photogenophores of Sinistroporomonorchis spp.: S glebulentus (A OQ672284/OQ674199, B OQ672285/OQ674200); S. mexicanus n. sp. (C OQ672296, D OQ672294); S. yucatanensis n. sp. (E OQ672297, F OQ672298/OQ674205); S. minutus n. sp. (G OQ672307, H OQ672310). Scale bars, 200 µm. In parentheses, GenBank accession numbers (see Table 1 for host and localities).
Figure 2
Figure 2
Alloinfundiburictus haemuli (Overstreet, 1969) Wee, Cutmore, Pérez-del-Olmo & Cribb, 2020 from Haemulon plumierii (A) Whole worm voucher; Scanning electron micrographs of voucher; (B) whole worm; (C) Oral sucker; (D) Ventral sucker; (E) Tegumental spines; (F) Posterior region. Scale bars (μm) = (A, B) 200; (C, D) 50; (E, F) 10.
Figure 3
Figure 3
Sinistroporomonorchis glebulentus (Overstreet, 1971) Wee, Cutmore, Pérez-del-Olmo & Cribb, 2020 from Mugil curema (A) Whole worm voucher; Scanning electron micrographs of voucher; (B) Whole worm; (C) Oral sucker; (D) Ventral sucker; (E) Tegumental spines at middle of body; (F) Tegumental spines at third of body; (G) Posterior region. Scale bars (μm) = (A, B) 200; (C) 40; (D) 50; (E, F) 5; (G) 20.
Figure 4
Figure 4
Sinistroporomonorchis mexicanus n. sp. from Mugil curema (A) Whole worm holotype, ventral view; (B) terminal genitalia of holotype; Scanning electron micrographs of voucher; (C) Whole worm; (D) Oral sucker; (E) Ventral sucker; (F) Tegumental spines; (G) Posterior region. Scale bars (μm) = (A, B, C) 100; (D, E) 40; (F) 5 μm; (G) 15.
Figure 5
Figure 5
Sinistroporomonorchis yucatanensis n. sp. from Mugil curema (A) Whole worm holotype, ventral view; (B) terminal genitalia of holotype; Scanning electron micrographs of voucher; (C) Whole worm; (D) Oral sucker; (E) Ventral sucker; (F) Tegumental spines; (G) Posterior region. Scale bars (μm) = (A) 200; (B, C) 100; (D) 40; (E) 50; (F) 5; (G) 30.
Figure 6
Figure 6
Sinistroporomonorchis minutus n. sp. from Mugil curema (A) Whole worm holotype, ventral view; (B) terminal genitalia of holotype; Scanning electron micrographs of voucher; (C) Whole worm; (D) Oral sucker; (E) Ventral sucker; (F) Tegumental spines; (G) Posterior region. Scale bars (μm) = (A) 100; (B) 50; (C) 100; (D) 30; (E) 50; (F) 5; (G) 20. Arrow marks genital pore.
Figure 7
Figure 7
Consensus Bayesian Inference and Maximum Likelihood trees inferred with LSU data set; numbers near internal nodes show posterior probabilities (BI) and ML bootstrap clade frequencies.
Figure 8
Figure 8
Consensus Bayesian Inference and Maximum Likelihood trees inferred with cox1 data set; numbers near internal nodes show posterior probabilities (BI) and ML bootstrap clade frequencies.
See this image and copyright information in PMC

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