Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 May 15;23(2):161.
doi: 10.1007/s10142-023-01070-8.

Reversal of H2O2-induced cell death by knockdown of HOTAIR in HTR-8/SVneo cells by mediation of miR-106b-5p/ACSL4 axis

Minkai Cao #  1 , Weilai Jin #  2 , Ying Li #  3 , Mingxin Wang  4 , Faguang Wan  5 , Yongwei Ren  6 , Ying Gu  7 , Jinqi Ma  8 , Le Zhang  9
Affiliations

Reversal of H2O2-induced cell death by knockdown of HOTAIR in HTR-8/SVneo cells by mediation of miR-106b-5p/ACSL4 axis

Minkai Cao et al. Funct Integr Genomics. .

Abstract

Preeclampsia is a serious threat to the health of pregnant women. Injury of trophoblasts could contribute to the progression of preeclampsia, and H2O2 was able to induce apoptosis in trophoblasts. LncRNAs have been reported to be involved in the progression of preeclampsia. Additionally, lncRNA HOTAIR is upregulated in patients with preeclampsia. However, the function of HOTAIR in H2O2-treated trophoblasts remains unclear. To explore the function of HOTAIR in preeclampsia, HTR-8/SVneo cells were stimulated with H2O2. RT-qPCR was performed to measure HOTAIR expression in HTR-8/SVneo cells. The apoptosis of HTR-8/SVneo cells was measured using TUNEL staining. The mitochondrial membrane potential was measured using JC-1 staining. Western blotting was performed to detect the expression of ACSL4, GPX4, and FTH1 in HTR-8/SVneo cells. The level of HOTAIR in HTR-8/SVneo cells was upregulated by H2O2. In addition, H2O2 notably inhibited the proliferation of HTR-8/SVneo cells, whereas knockdown of HOTAIR reversed this phenomenon. The mitochondrial membrane potential in HTR-8/SVneo cells was significantly inhibited by H2O2 and partially abolished by HOTAIR silencing. Moreover, HOTAIR could bind to miR-106b-5p; ACSL4 was identified as the downstream target of miR-106b-5p. Furthermore, HOTAIR knockdown reversed H2O2-induced ferroptosis in HTR-8/SVneo cells by regulating miR-106b-5p/ACSL4. Collectively, the knockdown of HOTAIR reversed H2O2-induced ferroptosis in HTR-8/SVneo cells by mediating miR-106b-5p/ACSL4. Thus, HOTAIR may serve as a new therapeutic target against preeclampsia.

Keywords: ACSL4; Ferroptosis; HOTAIR; Preeclampsia; miR-106b-5p.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Abildgaard U, Heimdal K (2013) Pathogenesis of the syndrome of hemolysis, elevated liver enzymes, and low platelet count (HELLP): a review. Eur J Obstet Gynecol Reprod Biol 166:117–123. https://doi.org/10.1016/j.ejogrb.2012.09.026 - DOI - PubMed
    1. Altman D, Carroli G, Duley L, Farrell B, Moodley J, Neilson J, Smith D (2002) Do women with pre-eclampsia, and their babies, benefit from magnesium sulphate? The Magpie Trial: a randomised placebo-controlled trial. Lancet 359:1877–1890. https://doi.org/10.1016/s0140-6736(02)08778-0 - DOI - PubMed
    1. Babic GM, Markovic SD, Varjacic M, Djordjevic NZ, Nikolic T, Stojic I, Jakovljevic V (2018) Estradiol decreases blood pressure in association with redox regulation in preeclampsia. Clin Exp Hypertens 40:281–286. https://doi.org/10.1080/10641963.2017.1368538 - DOI - PubMed
    1. Beharier O, Kajiwara K, Sadovsky Y (2021) Ferroptosis, trophoblast lipotoxic damage, and adverse pregnancy outcome. Placenta 108:32–38. https://doi.org/10.1016/j.placenta.2021.03.007 - DOI - PubMed - PMC
    1. Berzan E, Doyle R, Brown CM (2014) Treatment of preeclampsia: current approach and future perspectives. Curr Hypertens Rep 16:473. https://doi.org/10.1007/s11906-014-0473-5 - DOI - PubMed

LinkOut - more resources