Dehalogenimonas etheniformans sp. nov., a formate-oxidizing, organohalide-respiring bacterium isolated from grape pomace

Abstract

A strictly anaerobic, organohalide-respiring bacterium, designated strain GP^T, was characterized using a polyphasic approach. GP^T is Gram-stain-negative, non-spore-forming and non-motile. Cells are irregular cocci ranging between 0.6 and 0.9 µm in diameter. GP^T couples growth with the reductive dechlorination of 1,2-dichloroethane, vinyl chloride and all polychlorinated ethenes, except tetrachloroethene, yielding ethene and inorganic chloride as dechlorination end products. H₂ and formate serve as electron donors for organohalide respiration in the presence of acetate as carbon source. Major cellular fatty acids include C_{16 : 0}, C_{18 : 1}ω9c, C_{16 : 1}, C_{14 : 0} and C_{18 : 0}. On the basis of 16S rRNA gene phylogeny, GP^T is most closely related to Dehalogenimonas formicexedens NSZ-14^T and Dehalogenimonas alkenigignens IP3-3^T with 99.8 and 97.4 % sequence identities, respectively. Genome-wide pairwise comparisons based on average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization do not support the inclusion of GP^T in previously described species of the genus Dehalogenimonas with validly published names. On the basis of phylogenetic, physiological and phenotypic traits, GP^T represents a novel species within the genus Dehalogenimonas, for which the name Dehalogenimonas etheniformans sp. nov. is proposed. The type strain is GP^T (= JCM 39172^T = CGMCC 1.17861^T).

Keywords: Dehalococcoidia; Dehalogenimonas; organohalide respiration; reductive dechlorination; vinyl chloride.