The Relationship between Type 1 Diabetes Mellitus, TNF-α, and IL-10 Gene Expression

Abstract

Type 1 diabetes mellitus (T1DM) is one of the major chronic diseases in children worldwide. This study aimed to investigate interleukin-10 (IL-10) gene expression and tumor necrosis factor-alpha (TNF-α) in T1DM. A total of 107 patients were included, 15 were T1DM in ketoacidosis, 30 patients had T1DM and HbA1c ≥ 8%; 32 patients had T1DM and presented HbA1c < 8%; and 30 were controls. The expression of peripheral blood mononuclear cells was performed using the reverse transcriptase-polymerase chain reaction in real time. The cytokines gene expression was higher in patients with T1DM. The IL-10 gene expression increased substantially in patients with ketoacidosis, and there was a positive correlation with HbA1c. A negative correlation was found for IL-10 expression and the age of patients with diabetes, and the time of diagnosis of the disease. There was a positive correlation between TNF-α expression with age. The expression of IL-10 and TNF-α genes showed a significant increase in DM1 patients. Once current T1DM treatment is based on exogenous insulin, there is a need for other therapies, and inflammatory biomarkers could bring new possibilities to the therapeutic approach of the patients.

Keywords: HbA1c; gene expression; interleukin 10; ketoacidosis; tumor necrosis factor-α; type 1 diabetes mellitus.

Figure 1

Comparison of the median and interquartile range (box) of IL-10 RQ between groups. Different superscript letters indicate a significant difference between the means by the post hoc Holm–Sidak test for p-value < 0.050. Different superscript letters indicate a significant difference between the means by the post hoc Holm–Sidak test for p-value < 0.050.

Figure 2

Comparison of the mean and 95% confidence interval (error bar) of TNF RQ between groups. Different superscript letters indicate a significant difference between the means by the post hoc Holm–Sidak test for p-value < 0.050.