First report of whole genome sequence of septicemic Pasteurella multocida serovar B:2 'Soron' strain isolated from swine

Abstract

Pig pasteurellosis, caused by Pasteurella multocida, is an acute infection that also has economic implications for pig farmers. We report the complete genome sequence of a P. multocida, serovar B:2 'Soron' strain isolated from the blood of a pig that had died of pasteurellosis in India. The isolate was not found to be haemorrhagic septicaemia (HS) specific B:2 by the PCR assay. The genome of 'Soron' strain is a single circular chromosome of 2,272,124 base pairs in length and contains 2014 predicted coding regions, 4 ribosomal RNA operons, and 52 tRNAs. It has 1812 protein-coding genes that were also found in reference sequence PmP52Vac. Phylogenetic analysis revealed that Pm_P52VAc and P. multocida 'Soron' serovar B:2 were clustered in different clades. Pasteurella multocida 'Soron' serovar B:2 was found to cluster with the same ancestor of Pm70, which is of avian origin. The genome was found to contain regions that encode proteins which may confer resistance to various antibiotics including cephalosporin, which is used to treat pasteurellosis. The isolate was also found to harbour a phage region. This strain represents a novel multi-locus sequence type (MLST) that has not been previously identified, as all of the alleles used for MLST were found, but did not match any of the alleles in the database with 100% nucleotide identity. The most closely related ST was ST221. This is the first whole-genome sequence from P. multocida serovar B:2 of pig origin.

Keywords: Pasteurellosis; Pathogenicity; Serovar; Swine; WGS.

Fig. 1

Denovo assembly of P. multocida ‘Soron’ serovar B:2 strain

Fig. 2

Prediction of genes by COG, Uniprot, NR, and Pfam

Fig. 3

The gene distribution in different categories

Fig. 4

The local linear blocks generated from Mauve across P. multocida genomes

Fig. 5

The phylogenetic analysis of the 93 sequences showing Pm_P52VAC and P. multocida ‘Soron’ serovar B:2 clustered in different clades

Fig. 6

Comparison of a total of 237 and 193 genes using orthoMCL

Fig. 7

Assessment of the genome Pasteurella multocida which found encoding hypothetical proteins and 13 functional proteins

Fig. 8

PHASTER shows that the genome contains only one phage region that contains various phage proteins

Fig. 9

All three NIVEDI strains were found clustering together

Fig. 10

Some genomic regions in NIVEDI and SORON strains were either less or not identical with ATCC 43137 strain