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. 2023 May 16;18(5):e0280659.
doi: 10.1371/journal.pone.0280659. eCollection 2023.

Investigating the potential of packed cell volume for deducing hemoglobin: Cholistani camels in perspective

Umer Farooq  1 , Musadiq Idris  1 , Nouman Sajjad  1 , Mushtaq Hussain Lashari  2 , Shahbaz Ahmad  2 , Zia Ur Rehman  1 , Haroon Rashid  1 , Aisha Mahmood  1 , Sajid Hameed  3
Affiliations

Investigating the potential of packed cell volume for deducing hemoglobin: Cholistani camels in perspective

Umer Farooq et al. PLoS One. .

Abstract

In human medical practice, a hematological rule of three has been validated for healthy human populations. One such formula is estimating hemoglobin (Hb) levels as 1/3rd of Packed Cell Volume (PCV). However, no such hematological formulae have been devised and validated for veterinary medical practice. The present study was devised with an aim to evaluate the relationship between hemoglobin (Hb) concentration and Packed Cell Volume (PCV) in camels (n = 215) being reared under pastoralism, and to devise a simple pen-side hematological formula for estimation of Hb from PCV. The PCV was determined through microhematocrit method whereas Hb estimation by cyanmethaemoglobin method (HbD). The Hb was also calculated as 1/3rd of PCV and was dubbed as calculated Hb (HbC). Overall HbD and HbC were significantly (P≥0.05) different. Similar results were attained for all study groups i.e. males (n = 94) and females (n = 121), and young (n = 85) and adult (n = 130) camels. The corrected Hb (CHb) was deduced through regression prediction equation attained from linear regression model. Scatterplots were drawn, linear regression was carried out, and Bland Altman chart was built for agreement of both methods of Hb estimation. A non-significant (P≥0.05) difference was noticed between HbD and CHb. Bland Altman agreement analysis revealed satisfactory agreement between HbD and CHb and the data was distributed closely around the mean difference line (Mean = 0.1436, 95% CI = 3.00, -2.72). A simplified pen-side hematological formula for deducing Hb concentration from PCV is accordingly recommended viz. Hb concentration (g/dL) = 0.18(PCV)+5.4 for all age and gender groups of camels instead of its calculation as one-third of PCV.

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Conflict of interest statement

I have read the journal’s policy and the authors of this manuscript have the following competing interests it will helpful to manage the health of animals, and also helpful for professionals This does not alter our adherence to PLOS ONE policies on sharing data and materials.

Figures

Fig 1
Fig 1
Scatterplot for logilinear regression between a) hemoglobin determined spectrophotometrically (HbD) and Packed Cell Volume (PCV) (n = 215; r = 0.753), and b) between hemoglobin determined spectrophotometrically (HbD) and hemoglobin calculated as one-third of Packed Cell Volume (HbC) (n = 215; r = 0.753) in Cholistani camels.
Fig 2
Fig 2. Scatterplot of Bland and Altman test between difference of hemoglobin determined spectrophotometrically and corrected hemoglobin (HbD-CHbC) and average of both hemoglobins (HbD+CHb/2) in Cholistani camels (n = 215).
Black line indicates mean difference (0.1436) whereas the upper and lower red lines indicate upper (3.00) and lower (-2.72) values for 95% CI.
See this image and copyright information in PMC

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