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. 2023 Jul;67(14):e2200906.
doi: 10.1002/mnfr.202200906. Epub 2023 Jun 2.

Improving In Vitro Detection of Sensitization to Lipid Transfer Proteins: A New Molecular Multiplex IgE Assay

Balsells-Vives Sara  1 , Flügge Ulrike  2 , Brix Bettina  2 , Weimann Yvonne  2 , Peralta Teresa  3 , San Bartolomé Clara  1   4 , Araujo-Sánchez Giovanna  1   5 , Casas-Saucedo Rocío  1   5   6 , Torradeflot María  4 , Lara Rocío  4 , Munoz-Cano Rosa  1   5   6 , Bartra Joan  1   5   6   7 , Suer Waltraud  2 , Pascal Mariona  1   4   6   7
Affiliations
Free article

Improving In Vitro Detection of Sensitization to Lipid Transfer Proteins: A New Molecular Multiplex IgE Assay

Balsells-Vives Sara et al. Mol Nutr Food Res. 2023 Jul.
Free article

Abstract

Scope: LTP-syndrome is characterized by sensitization (IgE) to multiple non-specific lipid transfer proteins (nsLTPs) with a variable clinical outcome. The treatment is primarily based on offending food avoidance. However, the determination of Pru p 3-specific IgE is currently the main diagnostic tool to assess sensitization to nsLTPs. Herein, the study evaluates improvement of LTP-syndrome diagnosis and clinical management using a new IgE multiplex-immunoblot assay with a high diversity of food nsLTPs.

Methods and results: An EUROLINE-LTP strip with 28 recombinant nsLTPs from 18 allergenic sources is designed. In total the study investigates 38 patients with LTP-syndrome and compares results from the nsLTPs (LTP-strip) with the respective food extracts of Prick-by-prick (PbP) testing. The agreement exceeds 70% for most nsLTPs, e.g., Pru p 3 (100%), Mal d 3 (97%), Pru av 3 (89%), Pha v 3 isoforms (87%/84%), Ara h 9 (82%), Cor a 8 (82%), and Jug r 3 (82%). The functionality and allergenic relevance of nine recombinant nsLTPs are proven by Basophil activation testing (BAT).

Conclusions: The new IgE multiplex-immunoblot nsLTP assay shows a good diagnostic performance allowing culprit food assessment. Negative results from LTP-strip may indicate potentially tolerable foods, improving diet intervention and patients' quality of life.

Keywords: Basophil activation test; allergy; in vitro testing; lipid transfer proteins; multiplex immunoblot assay; plant food; serum specific IgE.

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