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. 2023 May 17;23(1):133.
doi: 10.1186/s12893-023-02037-z.

Indocyanine green combined with methylene blue versus methylene blue alone for sentinel lymph node biopsy in breast cancer: a retrospective study

Qiu-Hui Yang  1 , Xiang-Jian Zhang  2   3   4
Affiliations

Indocyanine green combined with methylene blue versus methylene blue alone for sentinel lymph node biopsy in breast cancer: a retrospective study

Qiu-Hui Yang et al. BMC Surg. .

Abstract

Background: Recent studies have shown that near-infrared (NIR) fluorescence imaging using Indocyanine green (ICG) may improve the efficiency of sentinel lymph node biopsy (SLNB). This study aimed to assess the effectiveness of the combination of ICG and methylene blue (MB) in breast cancer patients undergoing SLNB.

Patients and method: We evaluated ICG plus MB (ICG + MB) identification effectiveness with MB alone using retrospective analysis. From 2016 to 2020, we collected data on 300 eligible breast cancer patients who got SLNB treatment in our institution by ICG + MB or MB alone. By comparing the distribution of clinicopathological characteristics, the detection rate of sentinel lymph nodes (SLNs) and metastatic SLNs, as well as the total number of SLNs in the two groups, we were able to assess the imaging efficiency.

Results: Fluorescence imaging allowed 131 out of 136 patients in the ICG + MB group to find SLNs. ICG + MB group and MB group had detection rates of 98.5% and 91.5% (P = 0.007, χ2 = 7.352), respectively. Besides, the ICG + MB approach was able to produce improved recognition outcomes. What's more, compared with the MB group, the ICG + MB group can identify more lymph nodes (LNs) (3.1 to 2.6, P = 0.000, t = 4.447). Additionally, in the ICG + MB group, ICG could identify more LNs than MB (3.1 vs 2.6, P = 0.004, t = 2.884).

Conclusion: ICG has high detection effectiveness for SLNs, and when paired with MB, the detection efficiency can be increased even further. Furthermore, the ICG + MB tracing mode does not involve radioisotopes, which has a lot of promise for clinical use and can take the place of conventional standard detection methods.

Keywords: Breast cancer; Indocyanine green (ICG); Methylene blue (MB); Sentinel lymph node biopsy (SLNB); Sentinel lymph nodes (SLNs).

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
SLNs development status under ICG + MB A The development of SLNs observed by the naked eye; B The development state of the same SLNs on the left side under the fluorescence instrument, and the intraoperative records were as follows: ①B/G, ②B/G, ③B/G, ④B/G, ⑤G, ⑥G。
See this image and copyright information in PMC

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References

    1. Zhang LL, Ma L, Geng CZ, Jia Y, Wang XL, Liu YP. Correlation between extranodal invasion in axillary lymph node metastasis and prognosis of breast cancer patients. Zhonghua Bing Li Xue Za Zhi. 2017;46(8):525–529. - PubMed
    1. Krag DN, Anderson SJ, Julian TB, Brown AM, Harlow SP, Costantino JP, Ashikaga T, Weaver DL, Mamounas EP, Jalovec LM, et al. Sentinel-lymph-node resection compared with conventional axillary-lymph-node dissection in clinically node-negative patients with breast cancer: overall survival findings from the NSABP B-32 randomised phase 3 trial. Lancet Oncol. 2010;11(10):927–933. doi: 10.1016/S1470-2045(10)70207-2. - DOI - PMC - PubMed
    1. Giammarile F, Alazraki N, Aarsvold JN, Audisio RA, Glass E, Grant SF, Kunikowska J, Leidenius M, Moncayo VM, Uren RF, et al. The EANM and SNMMI practice guideline for lymphoscintigraphy and sentinel node localization in breast cancer. Eur J Nucl Med Mol Imaging. 2013;40(12):1932–1947. doi: 10.1007/s00259-013-2544-2. - DOI - PubMed
    1. Yang S, Xiang HY, Xin L, Zhang H, Zhang S, Cheng YJ, Liu Q, Xu L, Li T, Duan XN, et al. Retrospective analysis of sentinel lymph node biopsy using methylene blue dye for early breast cancer. Chin Med J (Engl) 2021;134(3):318–325. doi: 10.1097/CM9.0000000000001359. - DOI - PMC - PubMed
    1. OʼReilly EA, Prichard RS, Al Azawi D, Aucharaz N, Kelly G, Evoy D, Geraghty J, Rothwell J, OʼDoherty A, Quinn C, et al. The value of isosulfan blue dye in addition to isotope scanning in the identification of the sentinel lymph node in breast cancer patients with a positive lymphoscintigraphy: a randomized controlled trial (ISRCTN98849733) Ann Surg. 2015;262(2):243–248. doi: 10.1097/SLA.0000000000001213. - DOI - PubMed