Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Dec;124(3):1604-6.
doi: 10.1128/jb.124.3.1604-1606.1975.

Simple and sensitive procedure for screening yeast mutants that lyse at nonpermissive temperatures

E Cabib, A Duran

Simple and sensitive procedure for screening yeast mutants that lyse at nonpermissive temperatures

E Cabib et al. J Bacteriol. 1975 Dec.

Abstract

After mutagenesis, surviving yeast cells are grown on plates at 25 C and later exposed to 37 C. The plates are then overlaid with a soft agar containing p-nitrophenylphosphate at pH 9.7. Lysed cells liberate alkaline phosphatase which gives rise to a yellow color on and around colonies.

PubMed Disclaimer

References

    1. Biochim Biophys Acta. 1960 Jan 29;37:482-90 - PubMed
    1. Biochim Biophys Acta. 1963 Feb 12;67:324-6 - PubMed
    1. Genet Res. 1965 Feb;6:13-26 - PubMed
    1. Biochem Biophys Res Commun. 1965 Oct 26;21(2):182-6 - PubMed

LinkOut - more resources