Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986;42(1-2):105-9.
doi: 10.1159/000132260.

In situ hybridization of an acetylaminofluorene-modified probe recognized by Z-DNA antibodies in vitro

E Viegas-Péquignot, B Malfoy, M Leng, B Dutrillaux, P Tchen

In situ hybridization of an acetylaminofluorene-modified probe recognized by Z-DNA antibodies in vitro

E Viegas-Péquignot et al. Cytogenet Cell Genet. 1986.

Abstract

An in situ hybridization procedure, based on the chemical modification of DNA by acetylaminofluorene (AAF), followed by a specific immunoreaction was used to localize a Z-DNA sequence isolated from the satellite DNA of Cebus appella. The AAF probe is localized on the R-band-positive heterochromatic segments of Cebus chromosomes, which strongly react with Z-DNA antibodies. The use of a nonradioactive single-stranded labeled probe confirms the reliability and the rapidity of immunochemical methods for the detection of DNA sequences on chromosomes.

PubMed Disclaimer

Publication types