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[Preprint]. 2023 May 4:2023.05.04.539457.
doi: 10.1101/2023.05.04.539457.

Phosphorylation of 53BP1 by ATM enforce neurodevelopmental programs in cortical organoids

Bitna Lim, Mohamed Nadhir Djekidel, Yurika Matsui, Seunghyun Jung, Zuo-Fei Yuan, Xusheng Wang, Xiaoyang Yang, Abbas Shirinifard Pilehroud, Haitao Pan, Fang Wang, Shondra Pruett-Miller, Kanisha Kavdia, Vishwajeeth Pagala, Yiping Fan, Junmin Peng, Beisi Xu, Jamy C Peng

Phosphorylation of 53BP1 by ATM enforce neurodevelopmental programs in cortical organoids

Bitna Lim et al. bioRxiv. .

Update in

Abstract

53BP1 is a well-established DNA damage repair factor recently shown to regulate gene expression and critically influence tumor suppression and neural development. For gene regulation, how 53BP1 is regulated remains unclear. Here, we showed that 53BP1-serine 25 phosphorylation by ATM is required for neural progenitor cell proliferation and neuronal differentiation in cortical organoids. 53BP1-serine 25 phosphorylation dynamics controls 53BP1 target genes for neuronal differentiation and function, cellular response to stress, and apoptosis. Beyond 53BP1, ATM is required for phosphorylation of factors in neuronal differentiation, cytoskeleton, p53 regulation, and ATM, BNDF, and WNT signaling pathways for cortical organoid differentiation. Overall, our data suggest that 53BP1 and ATM control key genetic programs required for human cortical development.

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