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. 2023 Apr 17;20(6):725-736.
doi: 10.7150/ijms.82667. eCollection 2023.

Role of the gut microbiota and their metabolites in hemodialysis patients

Ying Ting Chao  1 , Ying-Kuang Lin  1   2 , Liang-Kun Chen  1 , Poyin Huang  3   4   5   6 , Yi-Chiung Hsu  1
Affiliations

Role of the gut microbiota and their metabolites in hemodialysis patients

Ying Ting Chao et al. Int J Med Sci. .

Abstract

High serum phosphate levels in chronic kidney disease (CKD) are linked to adverse health outcomes, including cardiovascular disease, kidney disease progression, and all-cause mortality. This study is aimed to find out which microorganisms or microbial functions have a significant impact on higher calcium-phosphorus product (Ca x P) after they undergo hemodialysis (HD) treatment. Feces samples from 30 healthy controls, 15 dialysis patients with controlled Ca xP (HD), and 16 dialysis patients with higher Ca xP (HDHCP) were collected to perform in 16S amplicon sequencing. We found gut microbial composition was significantly different between hemodialysis patients and healthy controls. Three phyla including Firmicutes, Actinobacteria, and Proteobacteria were significantly enriched in hemodialysis patients. Although only one genus, Lachnospiraceae_FCS020_group, was significantly increased in higher Ca xP group, there were four metabolic pathways predicted by PICRUSt significantly increased in higher Ca xP group and associated with causing VC, including the pentose phosphate pathway, steroid biosynthesis, terpenoid backbone biosynthesis, and fatty acid elongation pathway. Characterizing dysbiosis of gut microbiome played the important role in hemodialysis patients.

Keywords: calcium-phosphorus product; chronic kidney disease; dysbiosis; gut microbiota.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interest exists.

Figures

Figure 1
Figure 1
Gut microbial diversity based on OTUs distribution in healthy controls and hemodialysis patients (A-B).
Figure 2
Figure 2
Gut microbial composition of healthy controls and hemodialysis patients. Average compositions and relative abundance of the bacterial community in three groups at levels of the phylum (A) and the genus (B). (C) The difference in gut microbial composition was shown in Ctrl, HD and HDHCP.
Figure 3
Figure 3
Heatmap showing the relative abundance of the 68 genera that were significantly enriched between Ctrl, HD, and HDHCP.
Figure 3
Figure 3
Heatmap showing the relative abundance of the 68 genera that were significantly enriched between Ctrl, HD, and HDHCP.
Figure 4
Figure 4
Relative abundance of 5 genera with significantly enriched in HDHCP and average abundance more than 0.001 in Ctrl, HD and HDHCP.
Figure 5
Figure 5
The CCA analysis of the associations between the gut microbiome and clinical indicators for hemodialysis patients in the HD and HDHCP.
Figure 5
Figure 5
The CCA analysis of the associations between the gut microbiome and clinical indicators for hemodialysis patients in the HD and HDHCP.
Figure 6
Figure 6
Functional alterations of the gut microbiome in healthy controls and hemodialysis patients. LEfSe results showed a statistically significant increase in the abundance metabolism of KEGG pathways in the Ctrl, HD, and HDHCP.
See this image and copyright information in PMC

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