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Review
. 2023 Sep;71(9):2071-2095.
doi: 10.1002/glia.24390. Epub 2023 May 24.

Chemogenetic modulation of astrocytes and microglia: State-of-the-art and implications in neuroscience

Jo Bossuyt  1 , Yana Van Den Herrewegen  1 , Liam Nestor  1 , An Buckinx  1 , Dimitri De Bundel  1 , Ilse Smolders  1
Affiliations
Review

Chemogenetic modulation of astrocytes and microglia: State-of-the-art and implications in neuroscience

Jo Bossuyt et al. Glia. 2023 Sep.

Abstract

Insights into the role astrocytes and microglia play in normal and diseased brain functioning has expanded drastically over the last decade. Recently, chemogenetic tools have emerged as cutting-edge techniques, allowing targeted and spatiotemporal precise manipulation of a specific glial cell type. As a result, significant advances in astrocyte and microglial cell function have been made, showing how glial cells can intervene in central nervous system (CNS) functions such as cognition, reward and feeding behavior in addition to their established contribution in brain diseases, pain, and CNS inflammation. Here, we discuss the latest insights in glial functions in health and disease that have been made through the application of chemogenetics. We will focus on the manipulation of intracellular signaling pathways induced by activation of the designer receptors exclusively activated by designer drugs (DREADDs) in astrocytes and microglia. We will also elaborate on some of the potential pitfalls and the translational potential of the DREADD technology.

Keywords: CNS disorders; DREADDs; astrocytes; chemogenetics; designer receptors exclusively activated by designer drugs; gene therapy; microglia.

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References

REFERENCES

    1. Adamsky, A., Kol, A., Kreisel, T., Doron, A., Ozeri-Engelhard, N., Melcer, T., Refaeli, R., Horn, H., Regev, L., Groysman, M., London, M., & Goshen, I. (2018). Astrocytic activation generates de novo neuronal potentiation and memory enhancement. Cell, 174, 59-71.e14.
    1. Agalave, N. M., Larsson, M., Abdelmoaty, S., Su, J., Baharpoor, A., Lundbäck, P., Palmblad, K., Andersson, U., Harris, H., & Svensson, C. I. (2014). Spinal HMGB1 induces TLR4-mediated long-lasting hypersensitivity and glial activation and regulates pain-like behavior in experimental arthritis. Pain, 155(9), 1802-1813.
    1. Agulhon, C., Boyt, K. M., Xie, A. X., Friocourt, F., Roth, B. L., & McCarthy, K. D. (2013). Modulation of the autonomic nervous system and behaviour by acute glial cell Gq protein-coupled receptor activation in vivo. The Journal of Physiology, 591(22), 5599-5609.
    1. Agulhon, C., Fiacco, T. A., & McCarthy, K. D. (2010). Hippocampal short- and long-term plasticity are not modulated by astrocyte Ca2+ signaling. Science, 327(5970), 1250-1254.
    1. Airan, R. D., Thompson, K. R., Fenno, L. E., Bernstein, H., & Deisseroth, K. (2009). Temporally precise in vivo control of intracellular signalling. Nature, 458(7241), 1025-1029.

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