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Review
. 2023 Jul 20;61(7):e0023121.
doi: 10.1128/jcm.00231-21. Epub 2023 May 24.

Direct-from-Blood Detection of Pathogens: a Review of Technology and Challenges

Linoj Samuel  1
Affiliations
Review

Direct-from-Blood Detection of Pathogens: a Review of Technology and Challenges

Linoj Samuel. J Clin Microbiol. .

Abstract

Blood cultures have been the staple of clinical microbiology laboratories for well over half a century, but gaps remain in our ability to identify the causative agent in patients presenting with signs and symptoms of sepsis. Molecular technologies have revolutionized the clinical microbiology laboratory in many areas but have yet to present a viable alternative to blood cultures. There has been a recent surge of interest in utilizing novel approaches to address this challenge. In this minireview, I discuss whether molecular tools will finally give us the answers we need and the practical challenges of incorporating them into the diagnostic algorithm.

Keywords: PCR; blood culture; next-generation sequencing; sepsis.

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Conflict of interest statement

The authors declare a conflict of interest. The author has received grants/travel funding from Specific Diagnostics Inc and fees/travel funding from bioMerieux Inc.

References

    1. Wilson ML. 2020. Development of new methods for detecting bloodstream pathogens. Clin Microbiol Infect 26:319–324. doi: 10.1016/j.cmi.2019.08.002. - DOI - PubMed
    1. Weinstein MP, Towns ML, Quartey SM, Mirrett S, Reimer LG, Parmigiani G, Reller LB. 1997. The clinical significance of positive blood cultures in the 1990s: a prospective comprehensive evaluation of the microbiology, epidemiology, and outcome of bacteremia and fungemia in adults. Clin Infect Dis 24:584–602. doi: 10.1093/clind/24.4.584. - DOI - PubMed
    1. Wilson ML, Mirrett S, Reller LB, Weinstein MP, Reimer LG. 1993. Recovery of clinically important microorganisms from the BacT/Alert blood culture system does not require testing for seven days. Diagn Microbiol Infect Dis 16:31–34. doi: 10.1016/0732-8893(93)90127-s. - DOI - PubMed
    1. Yarbrough ML, Wallace MA, Burnham CD. 2021. Comparison of microorganism detection and time to positivity in pediatric and standard media from three major commercial continuously monitored blood culture systems. J Clin Microbiol 59:e00429-21. doi: 10.1128/JCM.00429-21. - DOI - PMC - PubMed
    1. Rhodes A, Evans LE, Alhazzani W, Levy MM, Antonelli M, Ferrer R, Kumar A, Sevransky JE, Sprung CL, Nunnally ME, Rochwerg B, Rubenfeld GD, Angus DC, Annane D, Beale RJ, Bellinghan GJ, Bernard GR, Chiche JD, Coopersmith C, De Backer DP, French CJ, Fujishima S, Gerlach H, Hidalgo JL, Hollenberg SM, Jones AE, Karnad DR, Kleinpell RM, Koh Y, Lisboa TC, Machado FR, Marini JJ, Marshall JC, Mazuski JE, McIntyre LA, McLean AS, Mehta S, Moreno RP, Myburgh J, Navalesi P, Nishida O, Osborn TM, Perner A, Plunkett CM, Ranieri M, Schorr CA, Seckel MA, Seymour CW, Shieh L, Shukri KA, et al. 2017. Surviving Sepsis Campaign: international guidelines for management of sepsis and septic shock: 2016. Intensive Care Med 43:304–377. doi: 10.1007/s00134-017-4683-6. - DOI - PubMed