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. 2023 Dec;133(12):3256-3268.
doi: 10.1002/lary.30762. Epub 2023 May 25.

Preclinical Models of Laryngeal Papillomavirus Infection: A Scoping Review

Renee E King  1   2   3 , Andrea Bilger  1 , Josef Rademacher  3 , Paul F Lambert  1 , Susan L Thibeault  3
Affiliations

Preclinical Models of Laryngeal Papillomavirus Infection: A Scoping Review

Renee E King et al. Laryngoscope. 2023 Dec.

Abstract

Objective: Laryngeal human papillomavirus (HPV) infection causes recurrent respiratory papillomatosis (RRP) and accounts for up to 25% of laryngeal cancers. Lack of satisfactory preclinical models is one reason that treatments for these diseases are limited. We sought to assess the literature describing preclinical models of laryngeal papillomavirus infection.

Data sources: PubMed, Web of Science, and Scopus were searched from the inception of database through October 2022.

Review methods: Studies searched were screened by two investigators. Eligible studies were peer-reviewed, published in English, presented original data, and described attempted models of laryngeal papillomavirus infection. Data examined included type of papillomavirus, infection model, and results including success rate, disease phenotype, and viral retention.

Results: After screening 440 citations and 138 full-text studies, 77 studies published between 1923 and 2022 were included. Models used low-risk HPV or RRP (n = 51 studies), high-risk HPV or laryngeal cancer (n = 16), both low- and high-risk HPV (n = 1), and animal papillomaviruses (n = 9). For RRP, 2D and 3D cell culture models and xenografts retained disease phenotypes and HPV DNA in the short term. Two laryngeal cancer cell lines were consistently HPV-positive in multiple studies. Animal laryngeal infections with animal papillomaviruses resulted in disease and long-term retention of viral DNA.

Conclusions: Laryngeal papillomavirus infection models have been researched for 100 years and primarily involve low-risk HPV. Most models lose viral DNA after a short duration. Future work is needed to model persistent and recurrent diseases, consistent with RRP and HPV-positive laryngeal cancer.

Level of evidence: NA Laryngoscope, 133:3256-3268, 2023.

Keywords: animal model; cell culture; larynx; papillomavirus; xenograft.

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Conflict of interest statement

Supported by the Diane M. Bless Endowed Chair, Division of Otolaryngology-Head & Neck Surgery, University of Wisconsin-Madison; the National Institute on Deafness and Other Communication Disorders (NIDCD) (grants R01 DC004336 and F31 DC018184); and the National Cancer Institute (NCI) (grants P01 CA022443, R35 CA210807, and T32 CA090217). The authors have no other relevant financial relationships or conflicts of interest to disclose.

Figures

Figure 1.
Figure 1.
Flow chart of study identification and screening as performed in accordance with the Preferred Reporting Items for Systematic Reviews extension for Scoping Reviews (PRISMA-ScR).
Figure 2.
Figure 2.
Studies included in the scoping review by decade of publication and laryngeal papillomavirus infection.
See this image and copyright information in PMC

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