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. 2023 May 11:48:109220.
doi: 10.1016/j.dib.2023.109220. eCollection 2023 Jun.

Data on plant defense enzyme activity associated with three endophytes against Cornus florida Erysiphe pulchra powdery mildew

Emily Rotich  1 , Margaret T Mmbaga  2
Affiliations

Data on plant defense enzyme activity associated with three endophytes against Cornus florida Erysiphe pulchra powdery mildew

Emily Rotich et al. Data Brief. .

Abstract

Three bacteria endophytes that colonize flowering dogwood (Cornus florida) suppressed Erysiphe pulchra powdery mildew disease severity. The three bacteria identified as Stenotrophomonas sp. (B17A), Serratia marcescens (B17B), and Bacillus thuringiensis (IMC8) were assessed for plant defense enzymes associated with plant protection. Detached leaves inoculated with powdery mildew were spray treated with the selected bacterial isolates and incubated for 15 h, 26 h, 48 h and 72 h and then analyzed for activation of defense enzymes and Pathogenesis related (PR) proteins associated with induced systemic resistance (ISR) as a potential mode of action against powdery mildew. At each time point post treatment with the bacteria, leaf tissue was ground in liquid nitrogen and stored at -70°C for biochemical assay of enzyme activity. This data set presents the activation of enzyme activity for peroxidase (PO), polyphenol oxidase (PPO) and β -1,3-glucanase at 15 h, 26 h, 48 h and 72 h post treatment with bacteria as indicated by a change in absorbance min -1 mg-1 per gram fresh weight of leaves. The gene expression of the corresponding pathogenesis related (PR) protein for each bacterial treatment compared to the control was also analyzed using Real time PCR and five primers targeting PR1, PR2, and PR5. While changes for PO, PPO, and β -1,3-glucanase enzyme activities were observed at different time points post treatment with all three bacteria, expression of PR protein was detected for PR1, but it was negligible for PR2, and PR5.

Keywords: Biological control; Disease management; Plant growth promotion; Reducing pesticides.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig 1
Fig. 1
Detached leaf treatments with three bacteria and nontreated control with five replicates and ten leaves per treatment in which B17A (Stenotrophomonas sp), B17B (Serratia marcescens), and IMC8 (Bacillus thuringiensis).
Fig. 2:
Fig. 2
Peroxidase (PO) activity expressed as change in absorbance at 480 nm per min per gram fresh weight of leaves. Control is non-treated, B17A (Stenotrophomonas sp.); B17B (Serratia marcescens), and IMC8 (Bacillus thuringiensis) at 3 × 109 CFU /ml and incubated for 15 h, 26 h, 48 h and 72 h after treatments.
Fig. 3:
Fig. 3
Polyphenol oxidase (PPO) activity expressed as change in absorbance at 480 nm per min per gram fresh weight of leaves. Control is non-treated, B17A (Stenotrophomonas sp.); B17B (Serratia marcescens), and IMC8 (Bacillus thuringiensis) at 3 × 109 CFU /ml and incubated for 15 h, 26 h, 48 h and 72 h after treatments.
Fig. 4:
Fig. 4
The mean β−1,3-glucanase activity expressed as change in absorbance at 480 nm per min per gram fresh weight of leaves. Control is non-treated, B17A (Stenotrophomonas sp.); B17B (Serratia marcescens), and IMC8 (Bacillus thuringiensis) at 3 × 109 CFU /ml, incubated for 15 h, 26 h, 48 h and 72 h after treatments.
Fig. 5
Fig. 5
The expression of PR1 genes shown by increases at 15, 26, 48 and 72 h post inoculation with B17A (Stenotrophomonas sp); B17B (Serratia marcescens), and IMC8 (Bacillus thuringiensis) and non-treated (Control).
See this image and copyright information in PMC

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