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. 2023 May;29(5):e13332.
doi: 10.1111/srt.13332.

Radiation therapy produces microvesicle particle release in HaCaT keratinocytes

Ericson John V Torralba  1 , Shikshita Singh  1 , Robert F Short  2 , Christine M Rapp  1 , Karen M Henkels  1 , Ravi P Sahu  1 , Jeffrey B Travers  1   3   4
Affiliations

Radiation therapy produces microvesicle particle release in HaCaT keratinocytes

Ericson John V Torralba et al. Skin Res Technol. 2023 May.
No abstract available

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Conflict of interest statement

None of the authors have a conflict of interest.

Figures

FIGURE 1
FIGURE 1
Ionizing radiation generates microvesicle particles (MVP) in HaCaT keratinocytes, which is blocked by an aSMase inhibitor. (A) HaCaT cells were subjected to ionizing radiation (IR) at various fluences, or treatment with 100 nM of the platelet‐activating factor receptor (PAFR) agonist carbamoyl PAF (CPAF) or the phorbol ester 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) or (0.1% ethanol) vehicle or nothing (sham). (B) HaCaT cells were pretreated with vehicle or 50 μM of the aSMase inhibitor imipramine, followed 1 h later with 4 Gy of IR. After 4 h, the supernatants were removed and MVP isolated and quantified and compared to cell numbers. The data in (A) are the mean  ± SD of MVP normalized to sham treatment and are representative of three separate experiments with similar findings. (B) Is mean ± SEM of three separate experiments. “*” Denotes statistically significant differences from sham‐treated values using Student's t‐test.
FIGURE 2
FIGURE 2
Ionizing radiation generates microvesicle particles (MVP) in KB epidermoid cells in a Platelet‐activating factor receptor (PAFR)‐independent manner. Human KB cells expressing the PAFR (KBP) or control retrovirally transduced PAFR‐negative (KBM) were subjected to ionizing radiation (IR) at various fluences, or treatment with 100 nM of the PAFR agonist carbamoyl PAF (CPAF), the phorbol ester 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA), or (0.1% ethanol) vehicle or nothing (sham). After 4 h, the supernatants were removed and MVP isolated and quantified and compared to cell numbers. The data are the mean ± SEM of MVP normalized to sham treatment from three separate experiments. “*” Denotes statistically significant differences from sham‐treated values using Student's t‐test.
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