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. 2023 Apr 27;21(5):274.
doi: 10.3390/md21050274.

Extraction, Characterization and Osteogenic Activity of a Type I Collagen from Starfish (Asterias amurensis)

Lingcui Li  1   2 , Yu Yu  3 , Wenhui Wu  1   2 , Peipei Wang  1   2
Affiliations

Extraction, Characterization and Osteogenic Activity of a Type I Collagen from Starfish (Asterias amurensis)

Lingcui Li et al. Mar Drugs. .

Abstract

Outbreaks of starfish (Asterias amurensis) pose a major threat to aquaculture and marine ecosystems in Qingdao, China, and no effective methods have been found to control them. A comprehensive study of collagen in starfish could be an alternative to high efficient utilization. Based on this, collagen was firstly extracted from Qingdao A. amurensis. Then, its protein pattern, amino acid composition, secondary structure, microstructure and thermal stability were investigated. The results showed that the A. amurensis collagen (AAC) is a type I collagen composed of α1, α2, and β chains. Glycine, hydroxyproline, and alanine were the major amino acids. The melting temperature was 57.7 °C. From FTIR, UV spectra and CD chromatography, the AAC had an intact triple helix and secondary structure, and microstructural analysis showed that the AAC had a loose, fibrous porous structure. Next, the osteogenic differentiation effect of AAC on Mouse bone marrow stem cells (BMSCs) was investigated, and the results showed that AAC induced osteogenic differentiation of cells by promoting the proliferation of BMSCs, enhancing alkaline phosphatase (ALP) activity, promoting cell mineralization nodules and upregulating the expression of mRNA of relevant osteogenic genes. These results suggest that AAC might have the potential application to bone health-related functional foods.

Keywords: Asterias amurensis; osteogenic differentiation; starfish; type I collagen.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
SDS-PAGE patterns of collagen from the skin of starfish. Lane 1: protein markers; lane 2: collagen from starfish.
Figure 2
Figure 2
(a) FTIR spectrum; (b) UV spectrum; (c) CD Spectral; (d) DSC.
Figure 3
Figure 3
Scanning electron microscopic structural image with different magnifications: (a) (10 µm), (b) (20 µm), (c) (100 µm).
Figure 4
Figure 4
(a) Effect of different concentrations of collagen on the proliferative capacity of BMSCs; (b) Effect of different concentrations of collagen on ALP activity; Effect of different concentrations of collagen on the relative expression of ALP gene (c), RUNX-2 gene (d), OPN gene (e) and BMP2 gene (f). * p < 0.05; ** p < 0.01; *** p < 0.001.
Figure 5
Figure 5
Effect of AAC on the generation of calcified nodules in BMSCs cells. (a) Induction for 7 days with induction solution; (b) Induction for 7 days with induction solution and collagen; (c) Induction for 16 days with induction solution; (d) Induction for 16 days with induction solution and collagen.
Figure 6
Figure 6
Schematic representation of steps involved in collagen extraction.
See this image and copyright information in PMC

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