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. 2023 May 19;16(5):768.
doi: 10.3390/ph16050768.

Effect of the Composition of Leuzea and Cranberry Meal Extracts on Metabolic Processes in Norm and Pathology

Daria Khalikova  1 , Sergey An'kov  1 , Nataliya Zhukova  1 , Tatyana Tolstikova  1 , Sergey Popov  1 , Anastasia Saiko  1
Affiliations

Effect of the Composition of Leuzea and Cranberry Meal Extracts on Metabolic Processes in Norm and Pathology

Daria Khalikova et al. Pharmaceuticals (Basel). .

Abstract

This study was conducted to evaluate the effects of long-term administration of a new herbal composition of leuzea and cranberry meal extracts at a dose of 70:500 mg/kg in healthy and pathological mice. After 4 weeks of daily composition administration to healthy CD-1 mice and C57BL/6 mice with diet-induced metabolic syndrome, oral glucose tolerance test (OGTT), serum biochemical examination and histology of internal organs were performed. Additionally, histological examination of white and brown adipose tissue was performed to evaluate the ability of the composition to prevent abdominal obesity in C57BL/6Ay (agouti yellow) mice. The results showed that the composition increased tissue sensitivity to glucose in healthy CD-1 mice; at the same time, it did not worsen the course of pathological processes in pathological mice. In both cases, the application of the developed composition was safe and contributed to the restoration of metabolic parameters.

Keywords: adipocytes; cranberry; ecdystene; extracts; leuzea; metabolic syndrome; mice; ursolic acid.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Figures

Figure 1
Figure 1
Area under the glycemic curve (AUC) calculated according to the oral glucose tolerance test (OGTT) data at the end of experiment, CD-1 mice. (A) Male; (B) Female. NC: negative control; Extr of leuzea: leuzea extract at a dose of 70 mg/kg; Extr of cranberry meal: cranberry meal extract at a dose of 500 mg/kg; the composition: composition of leuzea and cranberry meal extracts at a dose of 70:500 mg/kg. * p < 0.05 compared to Negative control; ** p < 0.005.
Figure 2
Figure 2
Body weight dynamics of C57BL/6 mice throughout the experiment (4 weeks). IC: intact control; NC: negative control; PC: positive control; Composition: composition of leuzea and cranberry meal extracts at a dose of 70:500 mg/kg. * p < 0.05 compared to negative control.
Figure 3
Figure 3
AUC calculated according to the OGTT data at the end of experiment, C57BL/6 mice. IC: intact control; NC: negative control; PC: positive control; Composition: composition of leuzea and cranberry meal extracts at a dose of 70:500 mg/kg. ** p < 0.005 compared to negative control; *** p < 0.0005. ## p < 0.005 compared to Intact control; ### p < 0.0005.
Figure 4
Figure 4
Histological evaluation of liver and pancreas in C57BL/6 mice at the end of experiment. (A) Intact control; (B) Negative control; (C) Composition: composition of leuzea and cranberry meal extracts at doses of 70:500 mg/kg; (D) Positive control: metformin at a dose of 250 mg/kg. Hematoxylin and eosin staining, ×200.
Figure 5
Figure 5
Histological evaluation of liver, brown adipose tissue (BAT), white adipose tissue (WAT) in C57BL/6Ay (AY) mice at the end of experiment. (A) Negative control; (B) Composition. Hematoxylin and eosin staining.
Figure 5
Figure 5
Histological evaluation of liver, brown adipose tissue (BAT), white adipose tissue (WAT) in C57BL/6Ay (AY) mice at the end of experiment. (A) Negative control; (B) Composition. Hematoxylin and eosin staining.
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