Study of Immune Response and Malondialdehyde Levels in Irradiated Rats Supplemented with Curcuma xanthorriza Roxb Extract

Abstract

Objective: The purpose of this study was to assess the immune response and malondialdehyde levels in irradiated rats supplemented with Curcuma xanthorriza Roxb extract as a candidate for mitigating radiation exposure.

Methods: Twenty-four male Wistar rats were grouped into eight treatment groups, then Curcuma xanthorrhiza Roxb extract was administered orally and irradiated at 6 Gy. Measurement of rats IL-6 and INF-γ was performed using a sandwich ELISA Kit, while the MDA concentration was quantified according to the method of Wills (1971). The statistical test is determined by one way ANOVA test. P-value <0.05 was considered statistically significant.

Result: The concentration of IL-6 in all groups showed no statistically significant difference (P=0.18). There was an increase in the concentration of IL-6 in the group of rats irradiated with 6 Gy for 7 days and 14 days. Meanwhile, the INF-γ concentration also showed no significant results in all treatment groups (P=0.28). The average of MDA concentration showed a significant difference in the liver and spleen of irradiated rats at 6 Gy for 14 days compared to the control (0.044 nmol/mg vs 0.008 nmol/mg, P=0.03 and 0.032 nmol/mg vs 0.014 nmol/mg, P=0.05, respectively).

Conclusion: The administration of Curcuma xanthorriza Xorb extract was able to reduce MDA concentrations in the liver and spleen although not statistically significant. In addition, exposure to ionizing radiation at a dose of 6 Gy significantly increased lipid peroxidation in the liver and spleen by 5.5 times and 2.3 times, respectively.

Keywords: Curcuma xanthorriza Roxb extract; Interferon-gamma; Interleukin-6; Malondialdehyde levels; radiation exposure.

Figure 1

Effects of Curcuma xanthorriza Xorb Extract (100 mg/kg body weight) and 6 Gy Irradiation Dose on the Value of IL-6. Comparisons were made using ANOVA followed by post-hoc Tukey's multiple comparison test (P-value=0.18). Note: P-value<0.05 was considered statistically significant

Figure 2

Effects of Curcuma xanthorriza Xorb Extract (100 mg/kg body weight) and 6 Gy Irradiation Dose on the Value of INF-γ (P-value=0.28)

Figure 3

Effects of Curcuma xanthorriza Xorb Extract (100 mg/kg body weight) and 6 Gy Irradiation Dose on the Value of MDA in Liver (P=0.03). Note: *P-value<0.05 was considered statistically significant

Figure 4

Effects of Curcuma xanthorriza Xorb Extract (100 mg/kg body weight) and 6 Gy Irradiation Dose on the Value of MDA in Spleen (P-value=0.05). Note: *P-value<0.05 was considered statistically significant