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. 2023 May 29;13(1):8668.
doi: 10.1038/s41598-023-35902-1.

Microsatellite markers reveal genetic diversity and population structure of Portunus trituberculatus in the Bohai Sea, China

Baohua Duan  1 , Tongxu Kang  1 , Haifu Wan  1 , Weibiao Liu  1 , Fenghao Zhang  1 , Shumei Mu  1 , Yueqiang Guan  1 , Zejian Li  2 , Yang Tian  3 , Xianjiang Kang  4   5   6
Affiliations

Microsatellite markers reveal genetic diversity and population structure of Portunus trituberculatus in the Bohai Sea, China

Baohua Duan et al. Sci Rep. .

Abstract

The swimming crab, Portunus trituberculatus, is one of the main aquaculture species in Chinese coastal regions due to its palatability and high economic value. To obtain a better understanding of the genetic diversity of P. trituberculatus in the Bohai Sea, the present study used 40 SSR loci to investigate the genetic diversity and population structure of 420 P. trituberculatus individuals collected from seven populations in the Bohai Sea. Genetic parameters revealed a low level of genetic diversity in the cultured population (SI = 1.374, He = 0.687, and PIC = 0.643) in comparison with wild populations (SI ≥ 1.399, He ≥ 0.692, and PIC ≥ 0.651). The genetic differentiation index (Fst) and gene flow (Nm) ranged from 0.001 to 0.060 (mean: 0.022) and 3.917 to 249.750 (mean: 31.289) respectively, showing a low differentiation among the seven populations of P. trituberculatus. Population structure analysis, phylogenetic tree, and principal component analysis (PCA) demonstrated that the seven groups of P. trituberculatus were divided into four subpopulations (K = 4), but the correlation between genetic structure and geographical distribution was not obvious. These results are expected to provide useful information for the fishery management of wild swimming crabs.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Swimming crab sampling locations. Note: This figure was created by DIVA-GIS 7.5 software (http://swww.diva-gis.org/).
Figure 2
Figure 2
Relationships between the number of clusters (K) and the corresponding Delta K statistics from structure analysis.
Figure 3
Figure 3
Population genetic structure based on the Bayesian clustering model among 420 P. trituberculatus individuals at K = 4.
Figure 4
Figure 4
The phylogenetic tree based on Nei's unbiased genetic distance (Nei, 1978) among 420 P. trituberculatus individuals.
Figure 5
Figure 5
The phylogenetic tree based on Nei's unbiased genetic distance (Nei, 1978) among seven P. trituberculatus populations.
Figure 6
Figure 6
Genetic relationships of 420 P. trituberculatus individuals as revealed by principal component analysis (PCA) with 40 SSR loci.
See this image and copyright information in PMC

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