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. 2023:2659:23-35.
doi: 10.1007/978-1-0716-3159-1_2.

Distinguishing Puccinia striiformis f. sp. tritici Isolates Using Genomic Sequencing: A Case Study

Michele Frick  1 , Eric Amundsen  1 , André Laroche  2
Affiliations

Distinguishing Puccinia striiformis f. sp. tritici Isolates Using Genomic Sequencing: A Case Study

Michele Frick et al. Methods Mol Biol. 2023.

Abstract

We are reporting on the utilization of high-throughput sequencing and different sequencing analysis tools to delineate identification of different isolates of the stripe rust fungal pathogen Puccinia striiformis f. sp. tritici (Pst). Different approaches are shown: utilization of rDNA sequences and random sequences that may be very useful to make sure that isolates belong to Pst and to distinguished closely related isolates. Identification of unique/lost sequences could lead to the identification of effectors associated with specific isolates.

Keywords: Bioinformatics; DNA isolation; High throughput sequencing; Polymorphisms; Puccinia striiformis f. sp. tritici; Random sequences; Wheat pathogen; rDNA sequences.

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