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. 2024 Jan;34(1):353-358.
doi: 10.1007/s10895-023-03278-0. Epub 2023 May 31.

A ThT Derivative as Zn2+ Sensor Based on DNA G-quadruplex

Xinyu Yuan  1 , Xiufeng Zhang  2   3 , Jinshan Hu  1 , Buyue Zhang  1 , Xiaoying Ma  1 , Jinxing He  1 , Lei Shi  1   4
Affiliations

A ThT Derivative as Zn2+ Sensor Based on DNA G-quadruplex

Xinyu Yuan et al. J Fluoresc. 2024 Jan.

Abstract

In this report, we developed a sensing strategy based on ThT-E (a ThT derivative) and DNA G-quadruplex for the label-free detection of Zn2+. In the absence of Zn2+, there was a fluorescence enhancement of ThT-E by interaction with human telomere sequence. On the addition of Zn2+, Zn2+ induced a more compact antiparallel G-quadruplex to release ThT-E, resulting in fluorescence quenching. The detection limit was 0.6996 μM, and the fluorescence intensity showed a good linear relationship with the concentration of Zn2+ in the range of 0-10 μM. This sensing strategy which only needs to mix two kinds of materials has the characteristics of label-feel, simple operation, short response time, economical and efficient.

Keywords: G-quadruplex; Label-free; Sensor; Thioflavin T derivative; Zn2+ detection.

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