Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Jul 18;12(7):e0033323.
doi: 10.1128/mra.00333-23. Epub 2023 Jun 1.

Complete Genome Sequence of Lopsy, a B1 Cluster Mycobacteriophage

Christopher Ealand #  1 , Edith Erika Machowski #  1 , Olivia Jacobs  1 , Bavesh Kana  1
Affiliations

Complete Genome Sequence of Lopsy, a B1 Cluster Mycobacteriophage

Christopher Ealand et al. Microbiol Resour Announc. .

Abstract

Lopsy is a siphovirus mycobacteriophage that is capable of lytic infection in Mycobacterium smegmatis. It is classified as a subcluster B1 mycobacteriophage and was isolated from soil in Estcourt, South Africa. The 68,542-bp double-stranded DNA genome is circularly permuted, has a GC content of 66.4%, and is predicted to contain 98 genes.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

FIG 1
FIG 1
Morphological characterization of mycobacteriophage Lopsy. (A) Petri dish (90 mm) containing solid culture medium (7H10) and bacterial host (Mycobacterium smegmatis mc2155) infected with Lopsy. Clear areas show the plaque morphology of Lopsy after incubation for 48 h at 37°C. Plaques appeared to be approximately 2 to 4 mm in diameter and clear, with no visible halo (scale bar = 10 mm). (B) Transmission electron micrograph of virion morphology (stained with 1% uranyl acetate). Lopsy contains an ~65-nm-wide head and a noncontractile tail with a length of ~290 nm (scale bar = 100 nm).
See this image and copyright information in PMC

References

    1. Aslam S, Lampley E, Wooten D, Karris M, Benson C, Strathdee S, Schooley RT. 2020. Lessons learned from the first 10 consecutive cases of intravenous bacteriophage therapy to treat multidrug-resistant bacterial infections at a single center in the United States. Open Forum Infect Dis 7:ofaa389. doi: 10.1093/ofid/ofaa389. - DOI - PMC - PubMed
    1. Azimi T, Mosadegh M, Nasiri MJ, Sabour S, Karimaei S, Nasser A. 2019. Phage therapy as a renewed therapeutic approach to mycobacterial infections: a comprehensive review. Infect Drug Resist 12:2943–2959. doi: 10.2147/IDR.S218638. - DOI - PMC - PubMed
    1. Hatfull GF, Dedrick RM, Schooley RT. 2022. Phage therapy for antibiotic-resistant bacterial infections. Annu Rev Med 73:197–211. doi: 10.1146/annurev-med-080219-122208. - DOI - PubMed
    1. Dedrick RM, Freeman KG, Nguyen JA, Bahadirli-Talbott A, Smith BE, Wu AE, Ong AS, Lin CT, Ruppel LC, Parrish NM, Hatfull GF, Cohen KA. 2021. Potent antibody-mediated neutralization limits bacteriophage treatment of a pulmonary Mycobacterium abscessus infection. Nat Med 27:1357–1361. doi: 10.1038/s41591-021-01403-9. - DOI - PMC - PubMed
    1. Guerrero-Bustamante CA, Dedrick RM, Garlena RA, Russell DA, Hatfull GF. 2021. Toward a phage cocktail for tuberculosis: susceptibility and tuberculocidal action of mycobacteriophages against diverse Mycobacterium tuberculosis strains. mBio 12:e00973-21. doi: 10.1128/mBio.00973-21. - DOI - PMC - PubMed

LinkOut - more resources