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. 2023 Aug;40(4):461-471.
doi: 10.1007/s10719-023-10119-6. Epub 2023 Jun 1.

Interactions between polysialic acid and dopamine-lead compounds as revealed by biochemical and in silico docking simulation analyses

Kaito Hayakawa  1   2 , Masaya Hane  1   2   3 , Hiroki Hamagami  4 , Miki Imai  4 , Hiroshi Tanaka  4 , Ken Kitajima  1   2   3 , Chihiro Sato  5   6   7
Affiliations

Interactions between polysialic acid and dopamine-lead compounds as revealed by biochemical and in silico docking simulation analyses

Kaito Hayakawa et al. Glycoconj J. 2023 Aug.

Abstract

Polysialic acid is an important glyco-epitope in vertebrate brains, while altered expressions of polySia and biosynthetic enzyme have been reported in brain diseases such as schizophrenia and depression. Recently, the binding between polySia and dopamine and the involvement of this in Akt signaling has been demonstrated. However, the molecular mechanism underlying the binding of polySia and dopamine remains unknown. Therefore, here, we demonstrated the interaction between dopamine and polySia using frontal affinity chromatography alongside docking simulations. In addition, we prepared dopamine-lead compounds to understand the detailed molecular basis of polySia binding by frontal affinity chromatography, enzyme-linked immunosorbent assay, and docking simulations.

Keywords: Docking simulation; Dopamine; Neurotransmitter; Polysialic acid; Sialic acid.

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