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. 2023 Jun 1;23(1):159.
doi: 10.1186/s12866-023-02889-8.

Genetic determinants of Biofilm formation of Helicobacter pylori using whole-genome sequencing

Kartika Afrida Fauzia  1   2   3 , Hafeza Aftab  4 , Muhammad Miftahussurur  3   5 , Langgeng Agung Waskito  3   6 , Vo Phuoc Tuan  7 , Ricky Indra Alfaray  1   3 , Takashi Matsumoto  1 , Michiyuki Yurugi  1 , Phawinee Subsomwong  8 , Evariste Tshibangu Kabamba  1   9 , Junko Akada  1 , Yoshio Yamaoka  10   11   12   13   14
Affiliations

Genetic determinants of Biofilm formation of Helicobacter pylori using whole-genome sequencing

Kartika Afrida Fauzia et al. BMC Microbiol. .

Abstract

Background: Infection with Helicobacter pylori as the cause of gastric cancer is a global public health concern. In addition to protecting germs from antibiotics, biofilms reduce the efficacy of H. pylori eradication therapy. The nucleotide polymorphisms (SNPs) related with the biofilm forming phenotype of Helicobacter pylori were studied.

Results: Fifty-six H. pylori isolate from Bangladeshi patients were included in this cross-sectional study. Crystal violet assay was used to quantify biofilm amount, and the strains were classified into high- and low-biofilm formers As a result, strains were classified as 19.6% high- and 81.4% low-biofilm formers. These phenotypes were not related to specific clades in the phylogenetic analysis. The accessories genes associated with biofilm from whole-genome sequences were extracted and analysed, and SNPs among the previously reported biofilm-related genes were analysed. Biofilm formation was significantly associated with SNPs of alpA, alpB, cagE, cgt, csd4, csd5, futB, gluP, homD, and murF (P < 0.05). Among the SNPs reported in alpB, strains encoding the N156K, G160S, and A223V mutations were high-biofilm formers.

Conclusions: This study revealed the potential role of SNPs in biofilm formation and proposed a method to detect mutation in biofilm from whole-genome sequences.

Keywords: Antibiotic resistance; Biofilm formation; Infectious disease; SNP; Variants; Whole-genome sequences.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
The morphology of biofilm formation on the coverslip surface on the day 4 of culture can be seen in this figure (10,000X magnification). The rod-shaped cells were covered with thick structure
Fig. 2
Fig. 2
The phylogenetic tree depicts the population and the biofilm formation The circle represents the population’s genetic type. The big circle with dark blue and light blue represented the high and low biofilm phenotype, respectively (grey: unknown because biofilm test was unavailable)
Fig. 3
Fig. 3
Scatter plot of P-values of the nucleotide polymorphisms (SNPs) in the target genes and their association with biofilm formation
See this image and copyright information in PMC

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