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. 2023 May 15;25(5):521-526.
doi: 10.7499/j.issn.1008-8830.2301031.

[Effect of procalcitonin on lipopolysaccharide-induced expression of nucleotide-binding oligomerization domain-like receptor protein 3 and caspase-1 in human umbilical vein endothelial cells]

[Article in Chinese]
Wen Jiang  1 , Ding-Hua Shi  1 , Yan-Juan He  1 , Chun-Yuan Chen  1
Affiliations

[Effect of procalcitonin on lipopolysaccharide-induced expression of nucleotide-binding oligomerization domain-like receptor protein 3 and caspase-1 in human umbilical vein endothelial cells]

[Article in Chinese]
Wen Jiang et al. Zhongguo Dang Dai Er Ke Za Zhi. .

Abstract
in English, Chinese

Objectives: To study the effect of procalcitonin (PCT) on lipopolysaccharide (LPS)-induced expression of the pyroptosis-related proteins nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) and caspase-1 in human umbilical vein endothelial cells (HUVECs).

Methods: HUVECs were induced by LPS to establish a model of sepsis-induced inflammatory endothelial cell injury. The experiment was divided into two parts. In the first part, HUVECs were randomly divided into four groups: normal control, LPS (1 μg/mL), PCT (10 ng/mL), and LPS+PCT (n=3 each). In the second part, HUVECs were randomly grouped: normal control, LPS, and LPS+PCT of different concentrations (0.1, 1, 10, and 100 ng/mL) (n=3 each). Quantitative real-time PCR and Western blot were used to measure the mRNA and protein expression levels of NLRP3 and caspase-1 in each group.

Results: In the first experiment: compared with the normal control group, the PCT, LPS, and LPS+PCT groups had significantly upregulated mRNA and protein expression levels of NLRP3 and caspase-1 (P<0.05); compared with the LPS group, the LPS+PCT group had significantly downregulated mRNA and protein expression levels of NLRP3 and caspase-1 (P<0.05). In the second experiment: compared with those in the LPS group, the mRNA and protein expression levels of NLRP3 and caspase-1 in the LPS+PCT of different concentrations groups were significantly downregulated in a concentration-dependent manner (P<0.05).

Conclusions: LPS can promote the expression of the pyroptosis-related proteins NLRP3 and caspase-1 in HUVECs, while PCT can inhibit the LPS-induced expression of the pyroptosis-related proteins NLRP3 and caspase-1 in HUVECs in a concentration-dependent manner.

目的: 探讨降钙素原（procalcitonin，PCT）对脂多糖（lipopolysaccharide，LPS）诱导的人脐静脉内皮细胞（human umbilical vein endothelial cells，HUVECs）焦亡相关蛋白核苷酸结合寡聚化结构域样受体蛋白3（nucleotide-binding oligomerization domain-like receptor protein 3，NLRP3）、半胱氨酸天冬氨酸蛋白酶-1（caspase-1）表达的影响。方法: 以LPS诱导HUVECs建立脓毒症内皮细胞炎症损伤模型。实验分为两部分：（1）将HUVECs随机分成正常对照组、LPS组（浓度1 μg/mL）、PCT组（浓度10 ng/mL）及LPS+PCT组（各组n=3）；（2）正常对照组、LPS组、LPS+PCT不同浓度（0.1、1、10、100 ng/mL）组（各组n=3）。采用实时荧光定量聚合酶链反应法和Western blot法检测各组细胞NLRP3、caspase-1 mRNA及其蛋白的表达。结果: （1）与正常对照组比较，LPS组、PCT组及LPS+PCT组NLRP3、caspase-1mRNA和蛋白表达均上调（P<0.05）；与LPS组比较，LPS+PCT组NLRP3、caspase-1 mRNA和蛋白表达均下调（P<0.05）。（2）与LPS组比较，LPS+PCT不同浓度组NLRP3、caspase-1 mRNA和蛋白表达下调；随PCT浓度增加，NLRP3、caspase-1 mRNA及蛋白表达逐渐下调（P<0.05）。结论: LPS可促进HUVECs焦亡相关因子NLRP3、caspase-1表达，PCT干预可抑制LPS诱导的HUVECs中焦亡相关蛋白NLRP3、caspase-1的表达，并呈浓度依赖性。.

Keywords: Caspase-1; Human umbilical vein endothelial cell; Nucleotide-binding oligomerization domain-like receptor protein 3; Procalcitonin; Pyroptosis; Sepsis.

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Figures

图1
图1. Western blot法检测各组NLRP3、caspase-1蛋白表达电泳图
图2
图2. Western blot法检测各组NLRP3、caspase-1蛋白表达电泳图
See this image and copyright information in PMC

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