Sectioning of Epon blocks in the 20 m to 60 micromicron range for histological studies

Abstract

Thick sections of tissue, (20 micron--60 micron), are useful in studying the relationship between individual large cells and cell layers in organized neural structures. The ability of the Nomarski Differential Interference-Contrast Microscope to bring a single thin layer into sharp focus makes the examination of such sections feasible. Although celloidin is the classical embedding medium for large, thick sections of neural tissue, the time necessary for this preparation is most inconvenient. Epon is an excellent embedding medium; however, it is extremely hard and brittle. By heating the Epon block face, thick sections can be cut. To avoid the cumbersome, often detrimental use of heat, a modification of this technique was found. Epon blocks, trimmed to a 1 millimeter square face, may be sectioned at room temperature on the sliding microtome at 20 micron to 60 micron with ease. The simple method of preparing such sections is described.