Correlation between the Shortened Period of Cell Pairing during Genomic Exclusion and the Block in Posttransfer Nuclear Development in Tetrahymena thermophia.: (conjugation/cell contact/star strains/nuclear transfer/Tetrahymena)

Abstract

The duration of pairing in crosses of the micronuclearly defective strain A^* V and the amicronuclear strain BI3840 of Tetrahymena thermophila is shorter than that in normal conjugation. In controls, pairing takes about 620 min at 30°C and 60 min more at 28°C. In contrast pairing of crosses of the A^* strain took 470 min at 30°C and 490 min at 28°C, and that of crosses of BI3840 strain took 440 min at 30°C. The course of nuclear development in the tester strain crossed with the A^* strain was similar to that in the control until the 3rd prezygotic division. Unilateral transfer of the pronuclei occurred later than on reciprocal transfer in control crosses; posttransfer divisions in hemikarya was completely blocked in over 90% of the cases examined. Defective cell contact in crosses of the A^* V strain and an amicronuclear BI3840 strain may be correlated with block in nuclear division and in macronuclear development, since unfertilized cells from triplet conjugation remain haploid and develop normally.