Elevated ozone alters long-chain fatty acids in leaves of Japanese white birch saplings

Abstract

Long-chain fatty acids (LCFAs) in leaves have attracted attention as nutritious phytochemicals and olfactory signals that influence the behavior and growth of herbivorous insects. In recognition of the negative effects of increasing tropospheric ozone (O₃) levels on plants, LCFAs can be altered through peroxidation by O₃. However, how elevated O₃ changes the amount and composition of LCFAs in field-grown plants is still unknown. We investigated palmitic, stearic, oleic, linoleic, linolenic LCFAs in the two leaf types (spring and summer) and two stages (early and late stage after expansion) of Japanese white birch (Betula platyphylla var. japonica) after a multi-year O₃ exposure on the field. Summer leaves exhibited a distinct composition of LCFAs under elevated O₃ at the early stage, whereas both stages of spring leaves did not exhibit significant changes in LCFAs composition by elevated O₃. In the spring leaves, the amounts of saturated LCFAs significantly increased at the early stage, however, the amount of total, palmitic, and linoleic acids at the late stage were significantly decreased by elevated O₃. Summer leaves had a lower amount of all LCFAs at both leaf stages. Regarding the early stage of summer leaves, the lower amount of LCFAs under elevated O₃ was possibly due to O₃-suppressed photosynthesis in the current spring leaves. Furthermore, the decrease ratio of spring leaves over time was significantly increased by elevated O₃ in all LCFAs, whereas summer leaves did not exhibit such an effect. These findings suggest that further studies should be conducted to reveal the biological functions of LCFAs under elevated O₃, considering the leaf type- and stage-dependent changes of LCFAs.

Keywords: Air pollution; Fatty acid; Ozone; Phenology; Plant–insect communication; Secondary metabolite.