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. 2023:30:20.
doi: 10.1051/parasite/2023022. Epub 2023 Jun 6.

Paradiplozoon cirrhini n. sp. (Monogenea, Diplozoidae), a gill parasite of Cirrhinus molitorella (Cyprinidae, Labeoninae) in South China

Jiayu Huang  1 , Xing Zhou  1 , Kai Yuan  2 , Xuejuan Ding  1
Affiliations

Paradiplozoon cirrhini n. sp. (Monogenea, Diplozoidae), a gill parasite of Cirrhinus molitorella (Cyprinidae, Labeoninae) in South China

Jiayu Huang et al. Parasite. 2023.

Abstract
in English, French

Paradiplozoon cirrhini n. sp. (Monogenea, Diplozoidae) is described from the gills of mud carp, Cirrhinus molitorella (Valenciennes, 1844) (Cyprinidae, Labeoninae), collected in Wuzhou, Guangxi Province, and Conghua, Guangdong Province as part of an ongoing survey of the diplozoid fauna in the Pearl River basin of China. The new Paradiplozoon species is distinguished from congeners by the structure of median plate and its outgrowth sclerites. The ITS2 sequences of the new species differ from all known available diplozoid sequences by 22.04%-38.34%. The new species is the first diplozoid species parasitic on Labeoninae in China. Molecular phylogenetic analyses using rRNA ITS2 placed Paradiplozoon cirrhini n. sp. in a sister position to the other Chinese Paradiplozoon, implying that Labeoninae represents an early and potentially ancestral host group for China Paradiplozoon. We also provided ITS2 sequences for four other diplozoids species, namely P. megalobramae Khotenovsky, 1982, P. saurogobionis (Jiang, et al., 1985) Jiang, Wu & Wang, 1989, Sindiplozoon hunanensis Yao & Wang, 1997, and Sindiplozoon sp., and validated their phylogenetic position. The results confirm that all diplozoid species are spilt into two major clades and show monophyly of Sindiplozoon but paraphyly of Paradiplozoon.

Title: Paradiplozoon cirrhini n. sp. (Monogenea, Diplozoidae), parasite des branchies de Cirrhinus molitorella (Cyprinidae, Labeoninae) dans le sud de la Chine.

Abstract: Paradiplozoon cirrhini n. sp. (Monogenea, Diplozoidae) est décrit à partir des branchies de la carpe de vase Cirrhinus molitorella (Valenciennes, 1844) (Cyprinidae, Labeoninae), collectée à Wuzhou, province du Guangxi, et à Conghua, province du Guangdong dans le cadre d’une enquête en cours sur la faune des Diplozoidae du bassin de la Rivière des Perles en Chine. La nouvelle espèce de Paradiplozoon se distingue de ses congénères par la structure de la plaque médiane et ses sclérites d’excroissance. Les séquences ITS2 de la nouvelle espèce diffèrent de toutes les séquences de Diplozoidae disponibles connues de 22,04 % à 38,34 %. La nouvelle espèce est la première espèce de Diplozoidae parasite de Labeoninae en Chine. Les analyses phylogénétiques moléculaires utilisant l’ARNr ITS2 ont placé Paradiplozoon cirrhini n. sp. dans une position sœur des autres Paradiplozoon chinois, ce qui implique que les Labeoninae représente un groupe d’hôtes précoce et potentiellement ancestral pour les Paradiplozoon de Chine. Nous avons également fourni des séquences ITS2 pour quatre autres espèces de Diplozoidae, à savoir P. megalobramae Khotenovsky, 1982, P. saurogobionis (Jiang, et al., 1985) Jiang, Wu & Wang, 1989, Sindiplozoon hunanensis Yao & Wang, 1997 et Sindiplozoon sp. et validé leur position phylogénétique. Les résultats confirment que toutes les espèces de Diplozoidae sont réparties en deux clades majeurs et montrent la monophylie de Sindiplozoon mais la paraphylie de Paradiplozoon.

Keywords: Diplozoidae; ITS2; New species; Paradiplozoon.

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Conflict of interest statement

All authors have no conflicts of interest. We acted in accordance with all applicable institutional and national laws and guidelines during this research.

Figures

Figure 1
Figure 1
Line drawings of Paradiplozoon cirrhini n. sp. A. Whole worm, ventral view (bs, buccal sucker; p, pharynx; e, esophagus; i, intestine; ld, lateral diverticula; o, ovary; t, testis; h, haptor; c, clamp); B. Clamp in somewhat lateral view (amp, anterior end of the median plate; pmp, posterior end of the median plate; mpj, medial sclerite of posterior jaw; lpj, lateral sclerite of posterior jaw; aj, anterior jaw; ajs, anterior joining sclerites; pjs, posterior joining sclerite); C. Clamp in posterior view; D. Clamp in anterior view; E. Central hook; F. Egg.
Figure 2
Figure 2
Line drawings of clamp sclerites of Paradiplozoon cirrhini n. sp. A. Medial sclerite of posterior jaw (mpj) and lateral sclerite of posterior jaw (lpj); B. Anterior jaw (aj) with a spur of the anterior jaw (saj); C. Median plate (C1, median plate in posterior view; C2, median plate in anterior view).
Figure 3
Figure 3
Photographs of Paradiplozoon cirrhini n. sp. A. Holotype, whole worm (ventral view); B. Reproductive system (te, testis; ov, ovary); C. Clamp in somewhat lateral view; D. Central hook; E. Eggs; F. Clamp sclerites in front view; G. Clamp sclerites in back view; H. Median plate in lateral view.
Figure 4
Figure 4
Photographs of clamps in different specimens of Paradiplozoon cirrhini n. sp. a–e are from adult worms, f is from a diporpa.
Figure 5
Figure 5
Mapping of the parasite geographical distribution onto the BI tree inferred from analyses of ITS2 sequences of selected diplozoids. The numbers at nodes indicate posterior probabilities (%); Paradiplozoon cirrhini n. sp are highlighted by red branches. Clades 1–5 represent the well-supported groups described in the “Results”.
Figure 6
Figure 6
Mapping of fish lineages onto the ML tree inferred from analyses of ITS2 sequences of selected diplozoids. The numbers at nodes indicate bootstrap values (%); Paradiplozoon cirrhini n. sp are highlighted by red branches.
See this image and copyright information in PMC

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