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1 Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA; Interdisciplinary Life Sciences Graduate Program, University of Texas at Austin, Austin, TX, USA; Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX, USA; LIVESTRONG Cancer Institute, Dell Medical School, University of Texas at Austin, Austin, TX, USA. Electronic address: dtaylor@utexas.edu.
1 Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA; Interdisciplinary Life Sciences Graduate Program, University of Texas at Austin, Austin, TX, USA; Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX, USA; LIVESTRONG Cancer Institute, Dell Medical School, University of Texas at Austin, Austin, TX, USA. Electronic address: dtaylor@utexas.edu.
Su et al.1 use a build-and-retrieve approach to both identify and determine structures of ten macromolecular machines in the human liver. The authors' method will launch researchers forward in understanding the structural biology of the cell (or organ).
Su. et. al (1) perform cryo-EM of human liver microsomes lysates and using their build-and-retrieve platform identify and determine 10 near-atomic structures of key macromolecular machines.
Aloy P; Böttcher B; Ceulemans H; Leutwein C; Mellwig C; Fischer S; Gavin A-C; Bork P; Superti-Furga G; Serrano L Structure-based assembly of protein complexes in yeast. Science 2004, 303, 2026–2029, DOI: 10.1126/science.1092645
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Ho C-M; Li X; Lai M; Terwilliger TC; Beck JR; Wohlschlegel J; Goldberg DE; Fitzpatrick AWP; Zhou ZH Bottom-up structural proteomics: cryoEM of protein complexes enriched from the cellular milieu. Nat. Methods 2020, 17, 79–85, DOI: 10.1038/s41592-019-0637-y
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Sae-Lee W, McCafferty CL, Verbeke EJ, Havugimana PC, Papoulas O, McWhite CD, Houser JR, Vanuytsel K, Murphy GJ, Drew K, Emili A, Taylor DW, Marcotte EM. The protein organization of a red blood cell. Cell Rep. 2022. Jul 19;40(3):111103. doi: 10.1016/j.celrep.2022.111103.
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-
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-
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Kastritis PL; O’Reilly FJ; Bock T; Li Y; Rogon MZ; Buczak K; Romanov N; Betts MJ; Bui KH; Hagen WJ Capturing protein communities by structural proteomics in a thermophilic eukaryote. Mol. Syst. Biol 2017, 13, 936, DOI: 10.15252/msb.20167412.
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