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. 1986 May 1;154(2):624-31.
doi: 10.1016/0003-2697(86)90038-2.

A highly sensitive assay method for human placental alkaline phosphatase involving a monoclonal antibody bound to a paper disk

K Hirano, Y Iiizumi, Y Hayashi, T Tanaka, M Sugiura, K Hayashi, Z D Lu, S Iino

A highly sensitive assay method for human placental alkaline phosphatase involving a monoclonal antibody bound to a paper disk

K Hirano et al. Anal Biochem. .

Abstract

A monoclonal antibody which is specific for human placental alkaline phosphatase and does not cross-react at all with intestinal alkaline phosphatase was prepared, and a procedure for the determination of placental alkaline phosphatase activity in serum was developed involving this monoclonal antibody bound to a paper disk. The minimum amount of placental alkaline phosphatase detectable by this method is 0.0025 King-Armstrong unit. Good correlation with the heat-treatment method was obtained. Therefore this proposed method can be used as a routine clinical test for the determination of serum placental alkaline phosphatase.

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