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. 1986 May;29(5):930-2.
doi: 10.1128/AAC.29.5.930.

Determination of aminoglycoside resistance in Staphylococcus aureus by DNA hybridization

N Dickgiesser, B N Kreiswirth

Determination of aminoglycoside resistance in Staphylococcus aureus by DNA hybridization

N Dickgiesser et al. Antimicrob Agents Chemother. 1986 May.

Abstract

A method is described for identification of the genes conferring aminoglycoside resistance in Staphylococcus aureus by dot-blot and Southern blot techniques. As radioactive probes, fragments of plasmids pAT48, pUBH2, and pH13, carrying the genes for an aminocyclitol-3'-phosphotransferase, an aminocyclitol-4'-adenylyltransferase, and an aminocyclitol-2''-phosphotransferase-aminocyclitol-6'-acetyltransferase, respectively, were used.

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References

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