Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Aug;55(8):1917-1929.
doi: 10.1007/s11255-023-03662-6. Epub 2023 Jun 9.

Exploring the characteristics of gut microbiome in patients of Southern Fujian with hypocitraturia urolithiasis and constructing clinical diagnostic models

Jialiang Wang #  1 , Guofeng Chen #  1 , Heyi Chen  1 , Jiabi Chen  1 , Qingfu Su  2 , Wei Zhuang  3
Affiliations

Exploring the characteristics of gut microbiome in patients of Southern Fujian with hypocitraturia urolithiasis and constructing clinical diagnostic models

Jialiang Wang et al. Int Urol Nephrol. 2023 Aug.

Abstract

Purpose: Hypocitraturia is an important cause of urolithiasis. Exploring the characteristics of the gut microbiome (GMB) of hypocitriuria urolithiasis (HCU) patients can provide new ideas for the treatment and prevention of urolithiasis.

Methods: The 24 h urinary citric acid excretion of 19 urolithiasis patients was measured, and patients were divided into the HCU group and the normal citrate urolithiasis (NCU) group. The 16 s ribosomal RNA (rRNA) was used to detect GMB composition differences and construct operational taxonomic units (OTUs) coexistence networks. The key bacterial community was determined by Lefse analysis, Metastats analysis and RandomForest analysis. Redundancy analysis (RDA) and Pearson correlation analysis visualized the correlation between key OTUs and clinical features and then established the disease diagnosis model of microbial-clinical indicators. Finally, PICRUSt2 was used to explore the metabolic pathway of related GMB in HCU patients.

Results: The alpha diversity of GMB in HCU group was increased and Beta diversity analysis suggested significant differences between HCU and NCU groups, which was related to renal function damage and urinary tract infection. Ruminococcaceae_ge and Turicibacter are the characteristic bacterial groups of HCU. Correlation analysis showed that the characteristic bacterial groups were significantly associated with various clinical features. Based on this, the diagnostic models of microbiome-clinical indicators in HCU patients were constructed with the areas under the curve (AUC) of 0.923 and 0.897, respectively. Genetic and metabolic processes of HCU are affected by changes in GMB abundance.

Conclusion: GMB disorder may be involved in the occurrence and clinical characteristics of HCU by influencing genetic and metabolic pathways. The new microbiome-clinical indicator diagnostic model is effective.

Keywords: Disease diagnostic model; Gut microbiome; Hypocitraturia; Urolithiasis.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Zuckerman JM, Assimos DG (2009) Hypocitraturia: pathophysiology and medical management. Rev Urol 11(3):134–144 - PubMed - PMC
    1. Sakhaee K et al (2011) Nephrolithiasis-associated bone disease: pathogenesis and treatment options. Kidney Int 79(4):393–403 - PubMed - DOI
    1. Arrabal-Polo MA et al (2013) Importance of citrate and the calcium : citrate ratio in patients with calcium renal lithiasis and severe lithogenesis. BJU Int 111(4):622–627 - PubMed - DOI
    1. Lan Y et al (2021) Glycine suppresses kidney calcium oxalate crystal depositions via regulating urinary excretions of oxalate and citrate. J Cell Physiol 236(10):6824–6835 - PubMed - DOI
    1. Osis G et al (2019) Regulation of renal NaDC1 expression and citrate excretion by NBCe1-A. Am J Physiol Renal Physiol 317(2):F489–F501 - PubMed - PMC - DOI

LinkOut - more resources