A one-step aptasensor for ultrasensitive detection of lung cancer marker homocysteine based on multifunctional carbon nanotubes by square-wave voltammetry

Abstract

In this work, a one-step aptasensor for ultrasensitive detection of homocysteine (HCY) is developed based on multifunctional carbon nanotubes, which is magnetic multi-walled carbon nanotubes (Fe₃O₄@MWCNTs) combined with the aptamer (Apt) for HCY (Fe₃O₄@MWCNTs-Apt). Fe₃O₄@MWCNTs-Apt have multiple functions as follows. (1) Apt immobilized could selectively capture all target molecules HCY in the sample; (2) Magnetic Fe₃O₄ nanoparticles could separate all target molecules HCY captured by Apt from the sample substrate to eliminate the background interference and achieve one-step preparation of the aptasensor; And (3), MWCNTs with good electrical conductivity become a new electrode surface, construct a three-dimensional electrode surface network, make the electron transfer easier and thus then enhance the signal response. Results show that there is a good linear relationship between peak current of square-wave voltammetry (SWV) and HCY concentration in the range of 0.01 μmol/L-1 μmol/L, with a limit of detection (LOD) 0.002 μmol/L. And, selectivity, reproducibility, precision and accuracy are all satisfactory. In addition, it could be applied to the detection of HCY in the plasma of lung cancer patients successfully, suggesting that this one-step aptasensor for HCY has a potential in practical clinical applications.

Keywords: Aptasensor; Homocysteine; Multifunctional carbon nanotubes; One-step; Square-wave voltammetry.